Document Detail

The pre-mRNA-splicing factor SF3a66 functions as a microtubule-binding and -bundling protein.
MedLine Citation:
PMID:  15142036     Owner:  NLM     Status:  MEDLINE    
SF3a (splicing factor 3a) complex is an essential component of U2 snRNPs (small nuclear ribonucleoprotein particles), which are involved in pre-mRNA splicing. This complex consists of three subunits: SF3a60, SF3a66 and SF3a120. Here, we report a possible non-canonical function of a well-characterized RNA-splicing factor, SF3a66. Ectopic expression experiments using each SF3a subunit in N1E 115 neuroblastoma cells reveals that SF3a66 alone can induce neurite extension, suggesting that SF3a66 functions in the regulation of cell morphology. A screen for proteins that bind to SF3a66 clarifies that SF3a66 binds to beta-tubulin, and also to microtubules, with high affinity, indicating that SF3a66 is a novel MAP (microtubule-associated protein). Electron microscopy experiments show that SF3a66 can bundle microtubules, and that bundling of microtubules is due to cross-bridging of microtubules by high-molecular-mass complexes of oligomerized SF3a66. These results indicate that SF3a66 is likely to be a novel MAP, and can function as a microtubule-bundling protein independently of RNA splicing.
Kei Takenaka; Hiroyuki Nakagawa; Shigeaki Miyamoto; Hiroaki Miki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  382     ISSN:  1470-8728     ISO Abbreviation:  Biochem. J.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-08-06     Completed Date:  2005-03-07     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  223-30     Citation Subset:  IM    
Division of Cancer Genomics, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
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MeSH Terms
COS Cells
Cell Line
Cell Line, Tumor
Cercopithecus aethiops
Microtubule-Associated Proteins / physiology*
Microtubules / metabolism
Neurites / metabolism
Neuroblastoma / chemistry,  pathology
Protein Binding / physiology
Protein Subunits / physiology
RNA Precursors / genetics*
RNA Splicing / physiology
Ribonucleoprotein, U2 Small Nuclear / physiology*
Ribonucleoproteins, Small Nuclear
Tubulin / metabolism
Reg. No./Substance:
0/Microtubule-Associated Proteins; 0/Protein Subunits; 0/RNA Precursors; 0/Ribonucleoprotein, U2 Small Nuclear; 0/Ribonucleoproteins, Small Nuclear; 0/Tubulin; 0/splicing factor 3a

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