| ppGpp accumulation in Pseudomonas aeruginosa and Pseudomonas fluorescens subjected to nutrient limitation and biocide exposure. | |
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MedLine Citation:
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PMID: 10664969 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of a commonly used biocide, 1,2-benzisothiazolin-3-one (BIT) on ppGpp accumulation in the pathogen, Pseudomonas aeruginosa PAO1, and an environmental isolate, Ps. fluorescens, was examined. It is concluded that BIT is able to induce a stringent response in Ps. aeruginosa and Ps. fluorescens, determined by the rapid accumulation of ppGpp following addition of BIT to exponentially-growing cells. Western blot analysis of whole-cell extracts with anti-RelA antibody demonstrated that both species contain a RelA homologue. This is the first report of a RelA-like protein in pseudomonads. |
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Authors:
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D L Greenway; R R England |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Letters in applied microbiology Volume: 29 ISSN: 0266-8254 ISO Abbreviation: Lett. Appl. Microbiol. Publication Date: 1999 Nov |
Date Detail:
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Created Date: 2000-02-28 Completed Date: 2000-02-28 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 8510094 Medline TA: Lett Appl Microbiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 298-302 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, University of Central Lancashire, Preston, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Western Culture Media Guanosine Tetraphosphate / metabolism* Microbial Sensitivity Tests Nitrogen / metabolism* Pseudomonas aeruginosa / drug effects*, growth & development, metabolism Pseudomonas fluorescens / drug effects*, growth & development, metabolism Reproducibility of Results Thiazoles / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Thiazoles; 2634-33-5/1,2-benzisothiazoline-3-one; 33503-72-9/Guanosine Tetraphosphate; 7727-37-9/Nitrogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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