Document Detail


ppGpp accumulation in Pseudomonas aeruginosa and Pseudomonas fluorescens subjected to nutrient limitation and biocide exposure.
MedLine Citation:
PMID:  10664969     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of a commonly used biocide, 1,2-benzisothiazolin-3-one (BIT) on ppGpp accumulation in the pathogen, Pseudomonas aeruginosa PAO1, and an environmental isolate, Ps. fluorescens, was examined. It is concluded that BIT is able to induce a stringent response in Ps. aeruginosa and Ps. fluorescens, determined by the rapid accumulation of ppGpp following addition of BIT to exponentially-growing cells. Western blot analysis of whole-cell extracts with anti-RelA antibody demonstrated that both species contain a RelA homologue. This is the first report of a RelA-like protein in pseudomonads.
Authors:
D L Greenway; R R England
Related Documents :
1108779 - Inhibtory effect of colicin e2 on transport systems of escherichia coli in the presence...
15797329 - Cell immobilization using self-assembled synthetic oligopeptide and its application to ...
1822839 - Cell aggregates of escherichia coli with benzylpenicillin amidase activity.
17868319 - Induction of apoptosis in caco-2 and ht-29 human intestinal epithelial cells by enteroh...
3298219 - Similarities in control of mini-f plasmid and chromosomal replication in escherichia coli.
20477899 - Rapid detection of escherichia coli in waters using fluorescent in situ hybridization, ...
24687429 - On the measurement of entropy production in living cells under an alternating electric ...
7507849 - Epithelial cells are an important source of tenascin in normal and malignant human brea...
17024559 - Thyroid hormone changes cardiomyocyte shape and geometry via erk signaling pathway: pot...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Letters in applied microbiology     Volume:  29     ISSN:  0266-8254     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  2000-02-28     Completed Date:  2000-02-28     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  298-302     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, University of Central Lancashire, Preston, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Culture Media
Guanosine Tetraphosphate / metabolism*
Microbial Sensitivity Tests
Nitrogen / metabolism*
Pseudomonas aeruginosa / drug effects*,  growth & development,  metabolism
Pseudomonas fluorescens / drug effects*,  growth & development,  metabolism
Reproducibility of Results
Thiazoles / pharmacology*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Thiazoles; 2634-33-5/1,2-benzisothiazoline-3-one; 33503-72-9/Guanosine Tetraphosphate; 7727-37-9/Nitrogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Cell surface display of hepatitis B virus surface antigen by using Pseudomonas syringae ice nucleati...
Next Document:  Selection of Salmonella typhimurium as an indicator for pathogen regrowth potential in composted bio...