Document Detail

ppGpp accumulation in Pseudomonas aeruginosa and Pseudomonas fluorescens subjected to nutrient limitation and biocide exposure.
MedLine Citation:
PMID:  10664969     Owner:  NLM     Status:  MEDLINE    
The effect of a commonly used biocide, 1,2-benzisothiazolin-3-one (BIT) on ppGpp accumulation in the pathogen, Pseudomonas aeruginosa PAO1, and an environmental isolate, Ps. fluorescens, was examined. It is concluded that BIT is able to induce a stringent response in Ps. aeruginosa and Ps. fluorescens, determined by the rapid accumulation of ppGpp following addition of BIT to exponentially-growing cells. Western blot analysis of whole-cell extracts with anti-RelA antibody demonstrated that both species contain a RelA homologue. This is the first report of a RelA-like protein in pseudomonads.
D L Greenway; R R England
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Letters in applied microbiology     Volume:  29     ISSN:  0266-8254     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  2000-02-28     Completed Date:  2000-02-28     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  298-302     Citation Subset:  IM    
Department of Biological Sciences, University of Central Lancashire, Preston, UK.
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MeSH Terms
Blotting, Western
Culture Media
Guanosine Tetraphosphate / metabolism*
Microbial Sensitivity Tests
Nitrogen / metabolism*
Pseudomonas aeruginosa / drug effects*,  growth & development,  metabolism
Pseudomonas fluorescens / drug effects*,  growth & development,  metabolism
Reproducibility of Results
Thiazoles / pharmacology*
Reg. No./Substance:
0/Culture Media; 0/Thiazoles; 2634-33-5/1,2-benzisothiazoline-3-one; 33503-72-9/Guanosine Tetraphosphate; 7727-37-9/Nitrogen

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