Document Detail


pp59fyn mutant mice display differential signaling in thymocytes and peripheral T cells.
MedLine Citation:
PMID:  1387588     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have generated mutant mice that do not express pp59fyn, a nonreceptor protein tyrosine kinase related to pp60src, by homologous recombination in embryonic stem cells. fyn- mice did not display an overt phenotype. Because fyn is associated with the T cell receptor (TCR), thymocyte and T cell signaling was analyzed in the mutant background. Cross-linking of TCR-CD3 in thymocytes led to markedly reduced calcium fluxes and abrogated proliferation, whereas mature splenic T cells retained largely normal proliferation despite depressed calcium movements and IL-2 production. Similarly, proliferation induced by Thy-1 cross-linking was reduced in thymocytes but not in splenic T cells. fyn- thymocytes were impaired at a late stage of maturation and showed limited clonal deletion to the Mls-1a self-super-antigen but not to staphylococcal enterotoxin A. These results implicate fyn as a critical component in TCR signaling in thymocytes and, potentially, in the process that determines T cell repertoire in the adult mouse.
Authors:
P L Stein; H M Lee; S Rich; P Soriano
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell     Volume:  70     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1992 Sep 
Date Detail:
Created Date:  1992-10-07     Completed Date:  1992-10-07     Revised Date:  2012-06-05    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  741-50     Citation Subset:  IM    
Affiliation:
Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies
Antigens, CD3
Antigens, Differentiation, T-Lymphocyte
Base Sequence
Cell Differentiation
Cells, Cultured
Ionomycin
Lymphocyte Activation*
Mice
Mice, Mutant Strains
Molecular Sequence Data
Phorbol Esters
Proto-Oncogene Proteins / physiology*
Proto-Oncogene Proteins c-fyn
Receptors, Antigen, T-Cell / physiology
Signal Transduction*
T-Lymphocytes / physiology*
Thymus Gland / cytology,  ultrastructure
Grant Support
ID/Acronym/Agency:
AI21420/AI/NIAID NIH HHS; HD24875/HD/NICHD NIH HHS; HD25326/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antigens, CD3; 0/Antigens, Differentiation, T-Lymphocyte; 0/Phorbol Esters; 0/Proto-Oncogene Proteins; 0/Receptors, Antigen, T-Cell; 56092-81-0/Ionomycin; EC 2.7.10.2/Fyn protein, mouse; EC 2.7.10.2/Proto-Oncogene Proteins c-fyn

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