Document Detail

The potential usefulness of free fetal DNA in maternal blood for prenatal fetal gender determination in multiple pregnancies.
MedLine Citation:
PMID:  22856355     Owner:  NLM     Status:  In-Data-Review    
We applied a noninvasive prenatal test for the determination of fetal gender in multiple pregnancies by using free fetal DNA circulating in maternal blood in order to evaluate whether the quantification of male DNA could distinguish the fetal gender and the number of male and female fetuses in multiple pregnancies. We enrolled consecutively 44 women with twin pregnancies between 11-14 weeks of gestation. Peripheral maternal blood was collected, and genomic DNA was extracted from maternal plasma and analyzed for the multicopy DYS 14 sequence by using real-time PCR to quantify male DNA. Results showed that male DNA concentration was significantly higher in twin pregnancies with at least one male fetus, compared to twin pregnancies with only female fetuses. Comparing male DNA concentration in pregnancies with two male fetuses versus pregnancies with one female fetus and one male fetus, we did not obtain a significant difference between the two groups due to a slight overlapping of the range values. Therefore, our test correctly predicted fetal gender, distinguishing twin pregnancies with at least one male fetus from twin pregnancies with only female fetuses, with a diagnostic accuracy of 100%. For distinguishing pregnancies with two male fetuses from pregnancies with both female and male fetuses, a diagnostic accuracy of 76.1% was achieved.
Elena Picchiassi; Gian Carlo Di Renzo; Federica Tarquini; Vittorio Bini; Michela Centra; Luana Pennacchi; Fabiana Galeone; Giuliana Coata
Related Documents :
23658325 - Isoleucine requirement of pregnant sows.
18975045 - Effects of maternal prenatal stress on offspring development: a commentary.
22881995 - How to read fetal heart rate tracings in labor. a comparison between acog and nice guid...
20925595 - Maternal omega-3 fatty acid supplementation and risk for perinatal maternal depression.
3517725 - Fetal biophysical profile in twin gestations.
12257225 - Herpes gestationis and repeated abortions.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Twin research and human genetics : the official journal of the International Society for Twin Studies     Volume:  15     ISSN:  1832-4274     ISO Abbreviation:  Twin Res Hum Genet     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-08-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101244624     Medline TA:  Twin Res Hum Genet     Country:  England    
Other Details:
Languages:  eng     Pagination:  143-8     Citation Subset:  IM    
Department of Obstetrics and Gynecology, University Hospital of Perugia, Perugia, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The use of nasal epithelial stem/progenitor cells to produce functioning ciliated cells in vitro.
Next Document:  Effects of chorionicity and zygosity on triplet birth weight.