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The potential of prolonged tissue culture to reduce stress generation and retraction in engineered heart valve tissues.
MedLine Citation:
PMID:  22889149     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In tissue engineered heart valves, cell-mediated processes cause tissue compaction during culture and leaflet retraction at time of implantation. We have quantified and correlated stress generation, compaction, retraction and tissue quality during a prolonged culture period of 8 weeks. PGA/P4HB strips were seeded with vascular-derived cells and cultured for 4 to 8 weeks. Compaction in width, generated force and stress were measured during culture. Retraction in length, generated force and stress were measured after release of constraints at week 4, 6 and 8. Further, the amount of DNA, GAG, collagen and collagen cross-links was assessed. During culture, compaction and force generation increased to respectively 63.9±0.8% and 43.7±4.3 mN at week 4, after which they remained stable. Stress generation reached 27.7±3.2 kPa at week 4, after which it decreased to approximately 8.5 kPa. At release of constraints, tissue retraction was 44.0±3.7% at week 4 and decreased to 29.2±2.8% and 26.1±2.2% at respectively 6 and 8 weeks. Generated force (8-16 mN) was lower at week 6 than at week 4 and 8. Generated stress decreased from 11.8±0.9 kPa at week 4 to 1.4±0.3 and 2.4±0.4 kPa at respectively week 6 and 8. The amount of GAGs increased at week 6 and 8 compared to week 4 and correlated to the reduced stress and retraction. In summary, prolonged culture resulted in decreased stress generation and retraction, likely as a result of the increased amount of GAGs. These results demonstrate the potential of prolonged tissue culture in developing functional, non-retracting, TE heart valves.
Authors:
Marijke A A van Vlimmeren; Anita Driessen-Mol; Cees Oomens; Frank Baaijens
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-14
Journal Detail:
Title:  Tissue engineering. Part C, Methods     Volume:  -     ISSN:  1937-3392     ISO Abbreviation:  Tissue Eng Part C Methods     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466663     Medline TA:  Tissue Eng Part C Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Eindhoven University of Technology, Department of Biomedical Engineering, Den Dolech 2, WH 4.12, Eindhoven, Netherlands, 5612AZ, +31402475415; marijkevanvlimmeren@gmail.com.
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