| The potential pattern of circulating lymphocytes TH1/TH2 is not altered after multiple injuries. | |
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MedLine Citation:
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PMID: 11074886 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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HYPOTHESIS: A shift in the balance of helper T cells type 1 (T(H)1) toward type 2 (T(H)2) has been suggested as a possible mechanism for impaired immune responses after severe trauma. We suggest that major injuries (polytrauma) induce an alteration in the pattern of T(H)1/T(H)2 cells. DESIGN, SETTING, AND PATIENTS: A prospective study of 35 polytraumatized patients (Injury Severity Score >16) admitted to a trauma intensive care unit at a level I trauma center (university hospital). INTERVENTIONS: Blood samples were collected from patients at various times during their stay in the intensive care unit and from age- and sex-matched healthy individuals. MAIN OUTCOME MEASURES: Serial determinations (n = 81) of intracellular interleukin (IL)-2 (T(H)1 cells) and IL-4 (T(H)2 cells) in stimulated CD3(+) T cells from patients with polytrauma twice a week during their stay in the intensive care unit accompanied by determination of the cell activation marker CD69 using 3-color flow cytometry. In parallel, the release of IL-2 and IL-4 from stimulated peripheral blood mononuclear cells and systemic plasma IL-4 levels were analyzed by conventional enzyme-linked immunosorbant assay. Healthy donors (n = 53) served as the control group. Data were related to outcome, Injury Severity Scores, and time after trauma. RESULTS: Expression of the cell activation marker CD69 was similar in stimulated lymphocytes from patients and healthy donors. There were no significant posttraumatic alterations in numbers of CD3(+) cells stained for intracellular IL-2 or IL-4, except for a minor decrease in IL-2(+) cells during the first week after trauma. Subgroups with high (>24) and lower (<25) Injury Severity Scores or survivors and nonsurvivors revealed no differences in intracellular cytokine staining. In contrast, patients revealed a highly significant decrease in the number of CD3(+) T cells. Mean systemic IL-4 levels did not differ in patients compared with healthy donors. Release of IL-2 and IL-4 from peripheral blood mononuclear cell fractions stimulated with phorbolmyristateacetate and ionomycin was significantly increased in patients with trauma but not from those stimulated with toxic shock syndrome toxin-1. CONCLUSIONS: Patients with multiple injuries have no significant alteration in the ratio of circulating T(H)1/T(H)2 cells. Thus, our results suggest pathomechanisms in posttraumatic T-cell suppression apart from alterations in the T(H)1/T(H)2 pattern. |
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Authors:
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M Wick; E Kollig; G Muhr; M Köller |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Archives of surgery (Chicago, Ill. : 1960) Volume: 135 ISSN: 0004-0010 ISO Abbreviation: Arch Surg Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2000-12-06 Completed Date: 2000-12-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9716528 Medline TA: Arch Surg Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1309-14 Citation Subset: AIM; IM |
Affiliation:
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Department of Surgery, BG Kliniken Bergmannsheil-Universitätsklinik, Bürkle-de-la-Camp-Platz 1, D-44789 Bochum, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD
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blood Antigens, Differentiation, T-Lymphocyte / blood Case-Control Studies Enzyme-Linked Immunosorbent Assay Female Flow Cytometry Humans Interleukin-2 / blood Interleukin-4 / blood Lymphocyte Activation Male Middle Aged Multiple Trauma / blood, immunology* Prospective Studies Th1 Cells / immunology* Th2 Cells / immunology* Trauma Severity Indices |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, Differentiation, T-Lymphocyte; 0/CD69 antigen; 0/Interleukin-2; 207137-56-2/Interleukin-4 |
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