| A potential oncogenic role of the commonly observed E2F5 overexpression in hepatocellular carcinoma. | |
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MedLine Citation:
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PMID: 21274376 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To explore the expression pattern of E2F5 in primary hepatocellular carcinomas (HCCs) and elucidate the roles of E2F5 in hepatocarcinogenesis. METHODS: E2F5 expression was analyzed in 120 primary HCCs and 29 normal liver tissues by immunohistochemistry analysis. E2F5-small interfering RNA was transfected into HepG2, an E2F5-overexpressed HCC cell line. After E2F5 knockdown, cell growth capacity and migrating potential were examined. RESULTS: E2F5 was significantly overexpressed in primary HCCs compared with normal liver tissues (P = 0.008). The E2F5-silenced cells showed significantly reduced proliferation (P = 0.004). On the colony formation and soft agar assays, the number of colonies was significantly reduced in E2F5-silenced cells (P = 0.004 and P = 0.009, respectively). E2F5 knockdown resulted in the accumulation of G0/G1 phase cells and a reduction of S phase cells. The number of migrating/invading cells was also reduced after E2F5 knockdown (P = 0.021). CONCLUSION: To our knowledge, this is the first evidence that E2F5 is commonly overexpressed in primary HCC and that E2F5 knockdown significantly repressed the growth of HCC cells. |
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Authors:
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Yuzhu Jiang; Seon-Hee Yim; Hai-Dong Xu; Seung-Hyun Jung; So Young Yang; Hae-Jin Hu; Chan-Kwon Jung; Yeun-Jun Chung |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: World journal of gastroenterology : WJG Volume: 17 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-28 Completed Date: 2011-07-18 Revised Date: 2011-07-21 |
Medline Journal Info:
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Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
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Languages: eng Pagination: 470-7 Citation Subset: IM |
Affiliation:
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Integrated Research Center for Genome Polymorphism and Department of Microbiology, School of Medicine, The Catholic University of Korea, Socho-gu, Seoul, South Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma, Hepatocellular
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genetics*,
metabolism*,
pathology Cell Cycle Cell Line, Tumor Cell Movement Cell Proliferation E2F5 Transcription Factor / genetics, metabolism* Gene Knockdown Techniques Humans Liver Neoplasms / genetics*, metabolism*, pathology Microarray Analysis Neoplasm Invasiveness Oncogenes* RNA, Small Interfering / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/E2F5 Transcription Factor; 0/E2F5 protein, human; 0/RNA, Small Interfering |
| Comments/Corrections | |
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