Document Detail

The potential of multimer technologies in type 1 diabetes prediction strategies.
MedLine Citation:
PMID:  21309048     Owner:  NLM     Status:  Publisher    
T1D is an autoimmune disease, which occurs in HLA genetically predisposed individuals as a consequence of the organ-specific immune destruction of the insulin-producing β cells in the islets of Langherans within the pancreas. T1D is the result of a breakdown in immune regulation that leads to expansion of autoreactive CD4+ and CD8+ T cells, autoantibody-producing B lymphocytes and activation of the innate immune system. Islet-related autoantibodies revealed themselves to be good predictors for future onset of the disease, although they are not directly pathogenetic; T cells instead play a dominant role in disease initiation and progression. In this review we first discuss the approaches that several laboratories attempted to measure human islet autoantigen-specific T cell function in T1D. T cell assays could be used in combination with standardized autoantibodies screenings to improve predictive strategies. They could also help to monitor in the long-term follow-up the efficacy of tolerogenic immunotherapeutic strategies when established at the onset of the disease, and help to predict the recurrence of disease. Although some recent developments based on ELISPOT and immunoblotting techniques have been able to distinguish with good sensitivity and specificity patients from controls, T cell results, as revealed by international workshops, were indeed largely inconclusive. Nowadays novel technologies have been exploited that could contribute to answering the 'tantalizing' question of identifying autoreactive T cells. We particularly focus on and discuss on MHC multimer tools and emphasize the advantages they can offer but also their weakness when used in combination with other T cells assays. Copyright © 2010 John Wiley & Sons, Ltd.
Alessandra Fierabracci
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-12-20
Journal Detail:
Title:  Diabetes/metabolism research and reviews     Volume:  -     ISSN:  1520-7560     ISO Abbreviation:  -     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2011-2-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883450     Medline TA:  Diabetes Metab Res Rev     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2010 John Wiley & Sons, Ltd.
Research Laboratories, Ospedale Pediatrico Bambino Gesù, Research Institute (IRCCS), Rome, Italy.
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