Document Detail

The potential of enzyme recycling during the hydrolysis of a mixed softwood feedstock.
MedLine Citation:
PMID:  19632826     Owner:  NLM     Status:  MEDLINE    
Despite recent improvement in cellulase enzymes properties, the high cost associated with the hydrolysis step remains a major impediment to the commercialization of full-scale lignocellulose-to-ethanol bioconversion process. As part of a research effort to develop a commercial process for bioconversion of softwood residues, we have examined the potential for recycling enzymes during the hydrolysis of mixed softwood substrate pretreated by organosolv process. We have used response surface methodology to determine the optimal temperature, pH, ionic strength, and surfactant (Tween 80) concentration for maximizing the recovery of bound protein and enzyme activity from the residual substrates after hydrolysis. Data analysis showed that the temperature, pH and surfactant concentration were the major factors governing enzyme desorption from residual substrate. The optimized conditions were temperature 44.4 degrees C, pH 5.3 and 0.5% Tween 80. The optimal conditions significantly increased the hydrolysis yield by 25% after three rounds of hydrolysis. This bound enzyme desorption combining with free enzyme re-adsorption is a potential method to recover cellulase enzymes and reduce the cost of enzymatic hydrolysis.
Maobing Tu; Xiao Zhang; Mike Paice; Paul MacFarlane; Jack N Saddler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-25
Journal Detail:
Title:  Bioresource technology     Volume:  100     ISSN:  1873-2976     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-08-31     Completed Date:  2009-11-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  6407-15     Citation Subset:  IM    
School of Forestry and Wildlife Sciences, Auburn University, Auburn, AL 36849-5418, USA.
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MeSH Terms
Adsorption / drug effects
Analysis of Variance
Cellulase / metabolism*
Conservation of Natural Resources*
Hydrogen-Ion Concentration / drug effects
Hydrolysis / drug effects
Lignin / metabolism
Models, Chemical
Osmolar Concentration
Plant Proteins / metabolism
Polysorbates / pharmacology
Wood / metabolism*
Reg. No./Substance:
0/Plant Proteins; 0/Polysorbates; 11132-73-3/lignocellulose; 9005-53-2/Lignin; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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