Document Detail


A potent sperm motility-inhibiting activity of bioflavonoids from an ethnomedicine of Onge, Alstonia macrophylla Wall ex A. DC, leaf extract.
MedLine Citation:
PMID:  15854639     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The methanol extract (ME) and the n-butanol fractions of methanolic extract of Alstonia macrophylla Wall ex A. DC leaves were investigated on the forward motility (FM) of mammalian (goat and human) spermatozoa. The ME at 600 microg mL-1 as well as fraction B at 100 microg mL-1 concentrations showed marked inhibition of sperm FM in both goat and human species when tested by microscopic and spectrophotometric methods. Approximately 60-80% of the goat spermatozoa lost their FM when treated with 600 microg mL-1 of ME and 100 microg mL-1 of fraction B. At 100 microg mL-1 concentration, fraction B showed 90% loss of FM in human spermatozoa, while fraction B at 400 microg mL-1 concentration showed complete inhibition of sperm FM at 0 min. The inhibitory activity of fraction B increases with increasing concentration in a dose-dependent manner. Phytochemical study of the extract revealed that the leaf contains tannins, flavonoids, sterols, triterpenes, alkaloids and reducing sugars. Further fractionation and purification of the bioactive n-butanol part of ME showed the presence of ursolic acid (fraction B), beta-sitosterol (fraction A), beta-sitosterol glucoside and a mixture of minor compounds (fraction C, detected on thin-layer chromatography). The results reveal that fraction B (ursolic acid), a pentacyclic triterpene, has the potential of sperm motility inhibition and can serve as a topical vaginal contraceptive.
Authors:
Debprasad Chattopadhyay; Sandhya R Dungdung; Asit B Mandal; Gopal C Majumder
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Contraception     Volume:  71     ISSN:  0010-7824     ISO Abbreviation:  Contraception     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-04-27     Completed Date:  2006-05-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0234361     Medline TA:  Contraception     Country:  United States    
Other Details:
Languages:  eng     Pagination:  372-8     Citation Subset:  IM    
Affiliation:
ICMR Virus Unit, Infectious Diseases and Beliaghata General Hospital, Beliaghata, Kolkata 700010, India. debprasadc@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Alstonia* / chemistry
Animals
Anti-Infective Agents / pharmacology*
Asia, Southeastern
Dose-Response Relationship, Drug
Flavonoids / pharmacology*
Goats
Humans
Male
Medicine, Traditional*
Phytotherapy*
Plant Extracts / chemistry,  pharmacology
Plant Leaves / chemistry
Plants, Medicinal / chemistry
Proteus / drug effects
Sperm Motility / drug effects*
Spermatocidal Agents / pharmacology*
Time Factors
Triterpenes / isolation & purification,  pharmacology*
Chemical
Reg. No./Substance:
0/Anti-Infective Agents; 0/Flavonoids; 0/Plant Extracts; 0/Spermatocidal Agents; 0/Triterpenes; 77-52-1/ursolic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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