| The post-translational trimethylation of diphthamide studied in vitro. | |
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MedLine Citation:
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PMID: 3346227 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The amino acid diphthamide is a complex post-translational derivative of histidine that exists in eukaryotic and Archaebacterial elongation factor 2 (EF-2). Diphtheria toxin and Pseudomonas exotoxin A catalyze the transfer of an ADP-ribose residue from NAD to diphthamide, causing the inactivation of EF-2. We have used cytosolic extracts of mutant CHO-K1 cells to study the biosynthesis of diphthamide in vitro. We have identified chromatographically a precursor form of diphthamide that exists in one complementation group of mutant cells and have documented the addition of 3 methyl residues from S-adenosylmethionine to this precursor. We have identified the presence of methyltransferase capable of carrying out this reaction in vitro in cells of 15 diverse eukaryotic species. |
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Authors:
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J M Moehring; T J Moehring |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 263 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1988 Mar |
Date Detail:
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Created Date: 1988-04-14 Completed Date: 1988-04-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3840-4 Citation Subset: IM |
Affiliation:
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Department of Microbiology, College of Agriculture and Life Sciences, University of Vermont, Burlington 05405. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Animals Cell Line Cytosol / metabolism Histidine / analogs & derivatives*, metabolism Methionine / metabolism Methylation Methyltransferases / metabolism* Peptide Elongation Factor 2 Peptide Elongation Factors / genetics* Protein Processing, Post-Translational* Species Specificity |
| Grant Support | |
ID/Acronym/Agency:
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AI-09100/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Peptide Elongation Factor 2; 0/Peptide Elongation Factors; 63-68-3/Methionine; 71-00-1/Histidine; 75645-22-6/diphthamide; EC 2.1.1.-/Methyltransferases; EC 2.1.1.-/diphthine methyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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