| A post-entry role for CD63 in early HIV-1 replication. | |
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MedLine Citation:
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PMID: 21315401 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Macrophages and CD4(+) lymphocytes are the major reservoirs for HIV-1 infection. CD63 is a tetraspanin transmembrane protein, which has been shown to play an essential role during HIV-1 replication in macrophages. In this study, we further confirm the requirement of CD63 in early HIV-1 replication events in both macrophages and a CD4(+) cell line. Further analysis revealed that viral attachment and cell-cell fusion were unaffected by CD63 silencing. However, CD63-depleted macrophages showed a significant decrease in the initiation and completion of HIV-1 reverse transcription, affecting subsequent events of the HIV-1 life cycle. Integration of HIV-1 cDNA as well as the formation of 2-LTR circles was notably reduced. Reporter assays showed that CD63 down regulation reduced production of the early HIV protein Tat. In agreement, CD63 silencing also inhibited production of the late protein p24. These findings suggest that CD63 plays an early post-entry role prior to or at the reverse transcription step. |
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Authors:
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Guangyu Li; Natallia Dziuba; Brian Friedrich; James L Murray; Monique R Ferguson |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-02-26 |
Journal Detail:
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Title: Virology Volume: 412 ISSN: 1096-0341 ISO Abbreviation: Virology Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-18 Completed Date: 2011-05-18 Revised Date: 2012-04-11 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 315-24 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Internal Medicine, Division of Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555-0435, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD
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metabolism* Antigens, CD63 CD4-Positive T-Lymphocytes / virology Cell Line HIV Core Protein p24 / biosynthesis HIV-1 / physiology* Humans Macrophages / virology Platelet Membrane Glycoproteins / metabolism* Reverse Transcription* Virus Integration Virus Internalization* Virus Replication tat Gene Products, Human Immunodeficiency Virus / biosynthesis |
| Grant Support | |
ID/Acronym/Agency:
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HL088999/HL/NHLBI NIH HHS; R01 HL088999-09/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, CD63; 0/CD63 protein, human; 0/HIV Core Protein p24; 0/Platelet Membrane Glycoproteins; 0/tat Gene Products, Human Immunodeficiency Virus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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