| A possible stimulatory effect of FMRFamide on neural nitric oxide production in the central nervous system of Helix lucorum L. | |
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MedLine Citation:
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PMID: 14673196 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The anatomical and functional relationship between neurons expressing nitric oxide (NO) synthase and molluscan cardioexcitatory (FMRFamide)-like neuropeptides was studied in the central ganglia of Helix lucorum (Pulmonata, Gastropoda), applying NADPHdiaphorase (NADPHd) histochemistry to visualize NO synthase and immunocytochemistry to demonstrate FMRFamide (FMRFa) at the light microscopic level. The NO production of the ganglia was detected by the colorimetric Griess determination of nitrite, a breakdown product of NO. Effects of the NO synthase substrate amino acid L-arginine, the NO synthase inhibitor Nomega-nitro-L-arginine (NOARG), synthetic FMRFa and the FMRFa sensitive ion channel blocker amiloride hydrochloride on nitrite production were also tested. NADPHd reaction labeled nerve cells and fibers in the procerebra, mesocerebra and metacerebra within the cerebral ganglia, and cell clusters in the postcerebral ganglia. FMRFa immunolabeling could be observed within subpopulations of NADPHd positive cells and in pericellular varicose fibers surrounding NADPHd stained neurons. Nitrite production of the ganglia was stimulated by L-arginine (10- 20 mM) but was decreased by NOARG (1-2 mM). Synthetic FMRFa (0.830-3.340 mM) increased the nitrite production in a dose dependent manner, but was ineffective in the presence of NOARG. Amiloride hydrochloride (7.890 mM) reduced the FMRFa evoked nitrite production in all ganglia. This is the first description of an anatomical relationship between putative NO producing and FMRFa containing cells, suggesting a possible regulatory role of FMRFa in the NO mediated signaling in an invertebrate nervous system. |
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Authors:
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Tamás Röszer; Zsolt Jenei; Tamás Gáll; Olivér Nagy; Zsolt Czimmerer; Zoltán Serfözö; Károly Elekes; Gáspár Bánfalvi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Brain, behavior and evolution Volume: 63 ISSN: 0006-8977 ISO Abbreviation: Brain Behav. Evol. Publication Date: 2004 |
Date Detail:
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Created Date: 2003-12-15 Completed Date: 2004-03-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0151620 Medline TA: Brain Behav Evol Country: Switzerland |
Other Details:
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Languages: eng Pagination: 23-33 Citation Subset: IM |
Copyright Information:
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Copyright 2004 S. Karger AG, Basel |
Affiliation:
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Department of Animal Anatomy and Physiology, Faculty of Natural Sciences, Debrecen University, Debrecen, Hungary. roszer@freemail.hu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals FMRFamide / metabolism* Ganglia, Invertebrate / cytology, enzymology Helix (Snails) / metabolism* Immunohistochemistry NADPH Dehydrogenase / metabolism Neurons / enzymology* Nitric Oxide / metabolism* Nitric Oxide Synthase / biosynthesis* Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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10102-43-9/Nitric Oxide; 64190-70-1/FMRFamide; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.6.99.1/NADPH Dehydrogenase |
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