Document Detail

A possible role for CD8(+) and non-CD8(+) cell granzyme B in early small airway wall remodeling in centrilobular emphysema.
MedLine Citation:
PMID:  23421932     Owner:  NLM     Status:  Publisher    
BACKGROUND AND OBJECTIVE: CD8(+) cell infiltration and apoptosis of airway epithelial cells is increased in COPD. CD8(+) T cells induce apoptosis by releasing granzymes, which can also cause extracellular matrix degradation and remodeling. Granzyme B levels and T cells expressing granzyme B are increased in BAL fluid of COPD patients, which suggests that granzyme B may contribute to the pathogenesis of COPD. This study provides quantitation of granzyme B positive cells in relation to CD8(+) cells in the small airway walls of emphysema. METHODS: Antibodies against CD8 and granzyme B were used to identify CD8(+) and granzyme B(+) cells. Volume fraction (Vv) of CD8(+) and granzyme B(+) cells were quantitated by the point counting method in the small airways of 13 nonsmoker control subjects and 46 emphysema patients (14 panlobular (PLE) and 32 centrilobular (CLE) emphysema lungs). Immunohistochemical detection of macrophage scavenger receptor was also performed in randomly selected cases. RESULTS: The volume fraction of CD8(+) and granzyme B(+) cells in CLE were greater than those in control and PLE (both p<0.001) subjects. The volume fraction of granzyme B(+) cells was greater than that of CD8(+) cells (p=0.006) and not all CD8(+) cells were positive for granzyme B in CLE subjects. Monocytes expressing both granzyme B and macrophage scavenger receptor and granulocytes expressing granzyme B were identified. CONCLUSIONS: Up-regulation of granzyme B in CD8(+) and non-CD8(+) cells is an early phenomenon of small airway wall remodeling in centrilobular emphysema and suggests its possible role in the pathogenesis of COPD.
Won-Dong Kim; Hyun-Sook Chi; Kang-Hyeon Choe; Yeon-Mok Oh; Sang-Do Lee; Kyu-Rae Kim; Kwang-Ha Yoo; David A Ngan; W Mark Elliott; Don D Sin; James C Hogg
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-20
Journal Detail:
Title:  Respirology (Carlton, Vic.)     Volume:  -     ISSN:  1440-1843     ISO Abbreviation:  Respirology     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9616368     Medline TA:  Respirology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Authors. Respirology © 2013 Asian Pacific Society of Respirology.
Division of Pulmonary Medicine, Konkuk University Medical Center.
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