| A possible explanation for the refractoriness of uterine prostaglandin production. | |
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MedLine Citation:
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PMID: 1995860 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Arachidonic acid increased the outputs of prostaglandin (PG) F-2 alpha, PGE-2 and 6-keto-PGF-1 alpha from the Day-7 and Day-15 guinea-pig uterus superfused in vitro. Similar increases in PG output were observed when the arachidonic acid treatment was repeated after an interval of 1, 3 or 5 h. Phospholipase (PL) A-2 increased the outputs of PGF-2 alpha, PGE-2 and 6-keto-PGF-1 alpha from the Day-7 guinea-pig uterus, but repeating the PLA-2 treatment 1 h later failed to stimulate PG output. The increase in outputs of PGF-2 alpha and PGE-2 caused by PLA-2 were partly restored after 3 h and were fully restored after 5 h, whereas the increase in 6-keto-PGF-1 alpha output produced by PLA-2 was only partly restored after 3 and 5 h. PLA-2 had little or no effect on PGF-2 alpha and PGE-2 outputs from the Day-15 guinea-pig uterus initially, and when repeated after 1, 3 and 5 h. This was probably due to the output of these two PGs, particularly of PGF-2 alpha, being stimulated in vivo before removal of the uterus. PLA-2 increased 6-keto-PGF-1 alpha output from the Day-15 uterus initially, but failed to cause a response when administered again 1 h later. After 3 and 5 h, the increase in 6-keto-PGF-1 alpha output from the Day-15 uterus caused by PLA-2 was partly restored. A23187 and PLC increased the outputs of PGF-2 alpha, PGE-2 and 6-keto-PGF-1 alpha from the Day-7 and Day-15 guinea-pig uterus. These responses to A23187 and PLC were reduced (but not abolished) when the two compounds were administered again 1 h later. After 3 and 5 h, the increases in output of PGF-2 alpha and PGE-2 produced by A23187 and PLC had returned to the initial values. The increases in output of 6-keto-PGF-1 alpha from the Day-7 and Day-15 guinea-pig uterus produced by A23187 and PLC were partly restored after 3 and 5 h, except for the response to PLC on Day 7 which was fully restored after 5 h. The results show that there is no failure with time in the mechanism which converts arachidonic acid into PGF-2 alpha in the guinea-pig uterus.(ABSTRACT TRUNCATED AT 400 WORDS) |
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Authors:
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N L Poyser |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of reproduction and fertility Volume: 91 ISSN: 0022-4251 ISO Abbreviation: J. Reprod. Fertil. Publication Date: 1991 Jan |
Date Detail:
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Created Date: 1991-03-26 Completed Date: 1991-03-26 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376367 Medline TA: J Reprod Fertil Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 371-84 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, University of Edinburgh Medical School, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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6-Ketoprostaglandin F1 alpha
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biosynthesis Animals Arachidonic Acids / pharmacology Calcimycin / pharmacology Dinoprost / biosynthesis* Dinoprostone / biosynthesis Female Guinea Pigs Organ Culture Techniques Perfusion Phospholipases A / pharmacology Stimulation, Chemical Time Factors Type C Phospholipases / pharmacology Uterus / drug effects, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Arachidonic Acids; 363-24-6/Dinoprostone; 52665-69-7/Calcimycin; 551-11-1/Dinoprost; 58962-34-8/6-Ketoprostaglandin F1 alpha; EC 3.1.1.-/Phospholipases A; EC 3.1.4.-/Type C Phospholipases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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