Document Detail


A possible explanation for the refractoriness of uterine prostaglandin production.
MedLine Citation:
PMID:  1995860     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Arachidonic acid increased the outputs of prostaglandin (PG) F-2 alpha, PGE-2 and 6-keto-PGF-1 alpha from the Day-7 and Day-15 guinea-pig uterus superfused in vitro. Similar increases in PG output were observed when the arachidonic acid treatment was repeated after an interval of 1, 3 or 5 h. Phospholipase (PL) A-2 increased the outputs of PGF-2 alpha, PGE-2 and 6-keto-PGF-1 alpha from the Day-7 guinea-pig uterus, but repeating the PLA-2 treatment 1 h later failed to stimulate PG output. The increase in outputs of PGF-2 alpha and PGE-2 caused by PLA-2 were partly restored after 3 h and were fully restored after 5 h, whereas the increase in 6-keto-PGF-1 alpha output produced by PLA-2 was only partly restored after 3 and 5 h. PLA-2 had little or no effect on PGF-2 alpha and PGE-2 outputs from the Day-15 guinea-pig uterus initially, and when repeated after 1, 3 and 5 h. This was probably due to the output of these two PGs, particularly of PGF-2 alpha, being stimulated in vivo before removal of the uterus. PLA-2 increased 6-keto-PGF-1 alpha output from the Day-15 uterus initially, but failed to cause a response when administered again 1 h later. After 3 and 5 h, the increase in 6-keto-PGF-1 alpha output from the Day-15 uterus caused by PLA-2 was partly restored. A23187 and PLC increased the outputs of PGF-2 alpha, PGE-2 and 6-keto-PGF-1 alpha from the Day-7 and Day-15 guinea-pig uterus. These responses to A23187 and PLC were reduced (but not abolished) when the two compounds were administered again 1 h later. After 3 and 5 h, the increases in output of PGF-2 alpha and PGE-2 produced by A23187 and PLC had returned to the initial values. The increases in output of 6-keto-PGF-1 alpha from the Day-7 and Day-15 guinea-pig uterus produced by A23187 and PLC were partly restored after 3 and 5 h, except for the response to PLC on Day 7 which was fully restored after 5 h. The results show that there is no failure with time in the mechanism which converts arachidonic acid into PGF-2 alpha in the guinea-pig uterus.(ABSTRACT TRUNCATED AT 400 WORDS)
Authors:
N L Poyser
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  91     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  1991 Jan 
Date Detail:
Created Date:  1991-03-26     Completed Date:  1991-03-26     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  371-84     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, University of Edinburgh Medical School, UK.
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MeSH Terms
Descriptor/Qualifier:
6-Ketoprostaglandin F1 alpha / biosynthesis
Animals
Arachidonic Acids / pharmacology
Calcimycin / pharmacology
Dinoprost / biosynthesis*
Dinoprostone / biosynthesis
Female
Guinea Pigs
Organ Culture Techniques
Perfusion
Phospholipases A / pharmacology
Stimulation, Chemical
Time Factors
Type C Phospholipases / pharmacology
Uterus / drug effects,  metabolism*
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 363-24-6/Dinoprostone; 52665-69-7/Calcimycin; 551-11-1/Dinoprost; 58962-34-8/6-Ketoprostaglandin F1 alpha; EC 3.1.1.-/Phospholipases A; EC 3.1.4.-/Type C Phospholipases

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