Document Detail

A possible correlation between DA-DAPI counterstaining and alu I-induced bands in fixed human chromosomes.
MedLine Citation:
PMID:  2424427     Owner:  NLM     Status:  MEDLINE    
DA-DAPI counterstaining is a technique which reveals the heterochromatic areas of chromosomes 1, 9, 15, 16 and Y in humans; a result which may be related to the peculiar characteristics of these chromosomal regions, particularly regarding their DNA content. The present report describes a different DA-DAPI stain reaction. Obtained under standard conditions, it shows positive, bright staining in a large number of chromosomes. Such a result is discussed in relation to the banding pattern produced by Alu I in human metaphase chromosomes, in view of the high degree of overlapping of the two patterns.
L Ferrucci; G F De Stefano; R Mezzanotte; R Vanni
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Basic and applied histochemistry     Volume:  30     ISSN:  0391-7258     ISO Abbreviation:  Basic Appl Histochem     Publication Date:  1986  
Date Detail:
Created Date:  1986-06-27     Completed Date:  1986-06-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7910664     Medline TA:  Basic Appl Histochem     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  61-8     Citation Subset:  IM    
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MeSH Terms
Chromosome Banding / methods*
DNA Restriction Enzymes / diagnostic use*
Deoxyribonucleases, Type II Site-Specific*
Distamycins / diagnostic use*
Indoles / diagnostic use*
Pyrroles / diagnostic use*
Staining and Labeling
Reg. No./Substance:
0/Distamycins; 0/Fixatives; 0/Indoles; 0/Pyrroles; 47165-04-8/DAPI; 636-47-5/stallimycin; EC 3.1.21.-/DNA Restriction Enzymes; EC 3.1.21.-/endodeoxyribonuclease AluI; EC, Type II Site-Specific

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