| The positive effect of the decreased NADPH-preferring activity of xylose reductase from Pichia stipitis on ethanol production using xylose-fermenting recombinant Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 17485825 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We focused on the effects of a mutation of xylose reductase from Pichia stipitis (PsXR) on xylose-to-ethanol fermentation using recombinant Saccharomyces cerevisiae transformed with PsXR and PsXDH (xylitol dehydrogenase from P. stipitis) genes. Based on inherent NADH-preferring XR and several site-directed mutagenetic studies using other aldo-keto reductase enzymes, we designed several single PsXR mutants. K270R showing decreased NADPH-preferring activity without a change in NADH-preferring activity was found to be a potent mutant. Strain Y-K270R transformed with K270R PsXR and wild-type PsXDH showed a 31% decrease in unfavorable xylitol excretion with 5.1% increased ethanol production as compared to the control in the fermentation of 15 g l(-1) xylose and 5 g l(-1) glucose. |
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Authors:
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Seiya Watanabe; Seung Pil Pack; Ahmed Abu Saleh; Narayana Annaluru; Tsutomu Kodaki; Keisuke Makino |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-05-07 |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 71 ISSN: 0916-8451 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2007 May |
Date Detail:
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Created Date: 2007-05-23 Completed Date: 2007-07-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 1365-9 Citation Subset: IM |
Affiliation:
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Faculty of Engineering, Kyoto University, Kyoto, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aldehyde Reductase
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genetics*,
metabolism Ethanol / metabolism* Fermentation Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Genes, Fungal Mutagenesis, Insertional NADP / metabolism* Pichia / genetics*, metabolism Recombination, Genetic Saccharomyces cerevisiae / genetics, metabolism* Xylose / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Xylose; 53-59-8/NADP; 64-17-5/Ethanol; EC 1.1.1.21/Aldehyde Reductase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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