Document Detail

The positive effect of the decreased NADPH-preferring activity of xylose reductase from Pichia stipitis on ethanol production using xylose-fermenting recombinant Saccharomyces cerevisiae.
MedLine Citation:
PMID:  17485825     Owner:  NLM     Status:  MEDLINE    
We focused on the effects of a mutation of xylose reductase from Pichia stipitis (PsXR) on xylose-to-ethanol fermentation using recombinant Saccharomyces cerevisiae transformed with PsXR and PsXDH (xylitol dehydrogenase from P. stipitis) genes. Based on inherent NADH-preferring XR and several site-directed mutagenetic studies using other aldo-keto reductase enzymes, we designed several single PsXR mutants. K270R showing decreased NADPH-preferring activity without a change in NADH-preferring activity was found to be a potent mutant. Strain Y-K270R transformed with K270R PsXR and wild-type PsXDH showed a 31% decrease in unfavorable xylitol excretion with 5.1% increased ethanol production as compared to the control in the fermentation of 15 g l(-1) xylose and 5 g l(-1) glucose.
Seiya Watanabe; Seung Pil Pack; Ahmed Abu Saleh; Narayana Annaluru; Tsutomu Kodaki; Keisuke Makino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  71     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-23     Completed Date:  2007-07-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1365-9     Citation Subset:  IM    
Faculty of Engineering, Kyoto University, Kyoto, Japan.
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MeSH Terms
Aldehyde Reductase / genetics*,  metabolism
Ethanol / metabolism*
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Fungal
Genes, Fungal
Mutagenesis, Insertional
NADP / metabolism*
Pichia / genetics*,  metabolism
Recombination, Genetic
Saccharomyces cerevisiae / genetics,  metabolism*
Xylose / metabolism*
Reg. No./Substance:
0/Xylose; 53-59-8/NADP; 64-17-5/Ethanol; EC Reductase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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