Document Detail


A portion of the human surfactant protein A (SP-A) gene locus consists of a pseudogene.
MedLine Citation:
PMID:  1827027     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
SP-A is the most abundant, surfactant-associated protein isolated from lung lavage. Genomic blot analysis of total human cellular DNA with SP-A cDNA demonstrated the presence of multiple hybridizing fragments that are not accounted for by available SP-A gene sequences. In this report, we have cloned and characterized human genomic DNA fragments that account for some of the other hybridizing fragments. These clones contain nucleotide sequences that are highly homologous to the fourth intron and fifth exon of the human SP-A gene. Sequences upstream from these SP-A-like sequences are not detectable by Northern blot hybridization of SP-A-expressing cells and the SP-A-like sequences contain premature stop codons, consistent with the interpretation that these clones represent an SP-A pseudogene. Restriction fragments consistent with this pseudogene and the functional SP-A gene are present in a human chromosome 10 genomic library made from a single chromosome, showing that the functional SP-A gene and the pseudogene are syntenic.
Authors:
T R Korfhagen; S W Glasser; M D Bruno; M J McMahan; J A Whitsett
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of respiratory cell and molecular biology     Volume:  4     ISSN:  1044-1549     ISO Abbreviation:  Am. J. Respir. Cell Mol. Biol.     Publication Date:  1991 May 
Date Detail:
Created Date:  1991-05-31     Completed Date:  1991-05-31     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8917225     Medline TA:  Am J Respir Cell Mol Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  463-9     Citation Subset:  IM    
Affiliation:
Division of Pulmonary Biology, Children's Hospital, Cincinnati, Ohio.
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MeSH Terms
Descriptor/Qualifier:
Bacteriophage lambda / genetics,  isolation & purification
Base Sequence
Chromosomes, Human, Pair 10 / chemistry*
DNA / analysis
Exons
Genomic Library
Humans
Introns
Lung / chemistry*
Molecular Sequence Data
Molecular Weight
Multigene Family
Proteolipids / genetics*
Pseudogenes*
Pulmonary Surfactant-Associated Protein A
Pulmonary Surfactant-Associated Proteins
Pulmonary Surfactants / genetics*
Restriction Mapping
Sequence Homology, Nucleic Acid
Grant Support
ID/Acronym/Agency:
HL-28623/HL/NHLBI NIH HHS; HL-41496/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Proteolipids; 0/Pulmonary Surfactant-Associated Protein A; 0/Pulmonary Surfactant-Associated Proteins; 0/Pulmonary Surfactants; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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