Document Detail


The polysialic acid units of the neural cell adhesion molecule N-CAM form filament bundle networks.
MedLine Citation:
PMID:  9786844     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polysialic acid is a developmentally regulated component in the neural cell adhesion molecule N-CAM which also occurs as the capsular polysaccharide of bacteria causing meningitis. Polysialic acid has been considered as a repulsive element that regulates intermolecular and intercellular adhesion. Using atomic force microscopy we unexpectedly find that oligomers of polysialic acid assemble with each other into filament bundle networks. Filaments were formed from oligomers containing 12 or more N-acetylneuraminic acid residues, and they were sensitive to sialidase digestion. The networks were also formed by the polysialic acid-containing carbohydrate units of N-CAM. The formation of filament bundles is a novel and unexpected property of polysialic acid and of short carbohydrate oligomers in general and represents a previously unrecognized molecular interaction mechanism which impacts both eukaryotic and prokaryotic cell-cell adhesions.
Authors:
J Toikka; J Aalto; J Häyrinen; L J Pelliniemi; J Finne
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  273     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1998-12-01     Completed Date:  1998-12-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  28557-9     Citation Subset:  IM    
Affiliation:
Department of Medical Biochemistry, University of Turku, FIN-20520 Turku, Finland.
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MeSH Terms
Descriptor/Qualifier:
Biopolymers
Cell Adhesion Molecules, Neuronal / metabolism*
Microscopy, Atomic Force
Sialic Acids / chemistry,  metabolism*
Chemical
Reg. No./Substance:
0/Biopolymers; 0/Cell Adhesion Molecules, Neuronal; 0/Sialic Acids; 0/polysialic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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