| The polysaccharide capsule of Campylobacter jejuni 81-176 modulates the host immune response. | |
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MedLine Citation:
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PMID: 23250948 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Campylobacter jejuni is a major cause of bacterial diarrheal disease worldwide. The organism is characterized by a diversity of polysaccharide structures, including a polysaccharide capsule. Most C. jejuni capsules are known to be decorated non-stoichiometrically with methyl phosphoramidate (MeOPN). The capsule of C. jejuni 81-176 has been shown to be required for serum resistance, but here we show that an encapsulated mutant lacking the MeOPN modification, mpnC, was equally sensitive to serum killing as the non-encapsulated mutant. A non-encapsulated mutant, kpsM, exhibited significantly reduced colonization compared to wildtype 81-176 in a mouse intestinal colonization model, and the mpnC mutant showed an intermediate level of colonization. Both mutants were associated with higher levels of IL-17 expression from lamina propria CD4+ cells compared to cells from animals infected with 81-176. In addition, reduced levels of TLR-4 and -2 activation were observed following in vitro stimulation of human reporter cell lines with kpsM and mpnC compared to wildtype 81-176. The data suggest that the capsule polysaccharide of C. jejuni, and the MeOPN modification, modulate the host immune response. |
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Authors:
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Alexander C Maue; Krystle L Mohawk; David K Giles; Frédéric Poly; Cheryl P Ewing; Yuening Jiao; Ginyoung Lee; Zuchao Ma; Mario A Monteiro; Christina L Hill; Jason S Ferderber; Chad K Porter; M Stephen Trent; Patricia Guerry |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-12-17 |
Journal Detail:
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Title: Infection and immunity Volume: - ISSN: 1098-5522 ISO Abbreviation: Infect. Immun. Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-12-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Enteric Diseases Dept., Naval Medical Research Center, Silver Spring, MD. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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