Document Detail

The polysaccharide capsule of Campylobacter jejuni modulates the host immune response.
MedLine Citation:
PMID:  23250948     Owner:  NLM     Status:  MEDLINE    
Campylobacter jejuni is a major cause of bacterial diarrheal disease worldwide. The organism is characterized by a diversity of polysaccharide structures, including a polysaccharide capsule. Most C. jejuni capsules are known to be decorated nonstoichiometrically with methyl phosphoramidate (MeOPN). The capsule of C. jejuni 81-176 has been shown to be required for serum resistance, but here we show that an encapsulated mutant lacking the MeOPN modification, an mpnC mutant, was equally as sensitive to serum killing as the nonencapsulated mutant. A nonencapsulated mutant, a kpsM mutant, exhibited significantly reduced colonization compared to that of wild-type 81-176 in a mouse intestinal colonization model, and the mpnC mutant showed an intermediate level of colonization. Both mutants were associated with higher levels of interleukin 17 (IL-17) expression from lamina propria CD4(+) cells than from cells from animals infected with 81-176. In addition, reduced levels of Toll-like receptor 4 (TLR4) and TLR2 activation were observed following in vitro stimulation of human reporter cell lines with the kpsM and mpnC mutants compared to those with wild-type 81-176. The data suggest that the capsule polysaccharide of C. jejuni and the MeOPN modification modulate the host immune response.
Alexander C Maue; Krystle L Mohawk; David K Giles; Frédéric Poly; Cheryl P Ewing; Yuening Jiao; Ginyoung Lee; Zuchao Ma; Mario A Monteiro; Christina L Hill; Jason S Ferderber; Chad K Porter; M Stephen Trent; Patricia Guerry
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-12-17
Journal Detail:
Title:  Infection and immunity     Volume:  81     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-21     Completed Date:  2013-04-18     Revised Date:  2013-09-03    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  665-72     Citation Subset:  IM    
Enteric Diseases Department, Naval Medical Research Center, Silver Spring, Maryland, USA.
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MeSH Terms
Campylobacter Infections / microbiology*
Campylobacter jejuni / physiology*
Cytokines / genetics,  metabolism
Gene Expression Regulation / immunology
HEK293 Cells
NF-kappa B / genetics,  metabolism
Polysaccharides, Bacterial / physiology*
Signal Transduction
Toll-Like Receptors / genetics,  metabolism
Grant Support
Reg. No./Substance:
0/Cytokines; 0/NF-kappa B; 0/Polysaccharides, Bacterial; 0/Toll-Like Receptors

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