Document Detail


A polyphasic approach to detect enterotoxigenic Staphylococcus aureus and diarrheagenic Escherichia coli in raw milk Italian cheeses by multiplex PCR.
MedLine Citation:
PMID:  21219749     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A polyphasic approach was evaluated for the detection of eight staphylococcal enterotoxin (SE)-encoding genes (sea, sec, sed, seg, seh, sei, sej, sel) and the Escherichia coli genes most commonly associated with virulence factors (eae, elt, ipaH, stx) in traditional soft cheeses, manufactured artisanally from whole raw milk in the Lombardy region (northern Italy). To determine the presence of the target genes, two multiplex PCRs were performed on DNA extracted both directly from cheese samples (culture-independent approach) and from whole cultivable cells, formed by harvesting colonies from the first serial dilution agar plates of selective media, as representative of cultivable community ("bulk"). Genes associated with enteroinvasive E. coli, ipaH, and Shiga toxin-producing E. coli, stx, were detected in two of the bulk samples analyzed; no virulence genes were found by amplifying DNA directly extracted from cheeses. SE-encoding genes were found in three cheeses (sea in all three samples, associated with sed and sej in two of these). More SE-encoding genes were detected by amplifying DNA obtained from bulk samples: sea, sed, sej, sec, seg, sel, and sei. No samples harbored the gene encoding for SE type H. The polyphasic approach followed has been useful in enhancing detection of target genes. Our results indicate that some of the artisanal cheeses examined may constitute a potential hazard for consumer health.
Authors:
Valentina Bernini; Elisa Sgarbi; Claudio Giorgio Bove; Monica Gatti; Erasmo Neviani
Related Documents :
8793399 - Detection of genes coding for extended-spectrum shv beta-lactamases in clinical isolate...
16949419 - Uterine transcriptomes of bacteria-induced and ovariectomy-induced preterm labor in mic...
16778369 - Association of caga+ helicobacter pylori with adenotonsillar hypertrophy.
20680279 - Identification of differentially expressed genes in flammulina velutipes with anti-tyro...
19471659 - Comparison between l and e gene amplification analytical methods for human papillomavir...
10574189 - Rapid method for relative gene expression determination in human tissues using automate...
7663429 - Developmental- and tissue-specific dna methylation patterns and expression of rat gamma...
23012479 - Impact of lactobacilli on orally acquired listeriosis.
15763669 - Cloning of dehydrin coding sequences from brassica juncea and brassica napus and their ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food protection     Volume:  73     ISSN:  1944-9097     ISO Abbreviation:  J. Food Prot.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2011-01-11     Completed Date:  2011-01-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2281-4     Citation Subset:  IM    
Affiliation:
Department of Genetics, Biology of Microorganisms, Anthropology, Evolution, University of Parma, Viale Usberti 11A, 43100, Parma, Italy. valentina.bernini@unipr.it
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cheese / microbiology*
Colony Count, Microbial
Consumer Product Safety
DNA, Bacterial / analysis
Enterotoxins / analysis,  genetics*
Escherichia coli / isolation & purification*
Female
Food Contamination / analysis*
Food Microbiology
Gene Amplification
Genotype
Humans
Milk / microbiology
Polymerase Chain Reaction*
Prevalence
Staphylococcus aureus / isolation & purification*
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/Enterotoxins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Natural-light labeling of tomatoes does not facilitate growth or penetration of Salmonella into the ...
Next Document:  Emergence and characterization of foodborne methicillin-resistant Staphylococcus aureus in Korea.