Document Detail

A polyphasic approach to detect enterotoxigenic Staphylococcus aureus and diarrheagenic Escherichia coli in raw milk Italian cheeses by multiplex PCR.
MedLine Citation:
PMID:  21219749     Owner:  NLM     Status:  MEDLINE    
A polyphasic approach was evaluated for the detection of eight staphylococcal enterotoxin (SE)-encoding genes (sea, sec, sed, seg, seh, sei, sej, sel) and the Escherichia coli genes most commonly associated with virulence factors (eae, elt, ipaH, stx) in traditional soft cheeses, manufactured artisanally from whole raw milk in the Lombardy region (northern Italy). To determine the presence of the target genes, two multiplex PCRs were performed on DNA extracted both directly from cheese samples (culture-independent approach) and from whole cultivable cells, formed by harvesting colonies from the first serial dilution agar plates of selective media, as representative of cultivable community ("bulk"). Genes associated with enteroinvasive E. coli, ipaH, and Shiga toxin-producing E. coli, stx, were detected in two of the bulk samples analyzed; no virulence genes were found by amplifying DNA directly extracted from cheeses. SE-encoding genes were found in three cheeses (sea in all three samples, associated with sed and sej in two of these). More SE-encoding genes were detected by amplifying DNA obtained from bulk samples: sea, sed, sej, sec, seg, sel, and sei. No samples harbored the gene encoding for SE type H. The polyphasic approach followed has been useful in enhancing detection of target genes. Our results indicate that some of the artisanal cheeses examined may constitute a potential hazard for consumer health.
Valentina Bernini; Elisa Sgarbi; Claudio Giorgio Bove; Monica Gatti; Erasmo Neviani
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food protection     Volume:  73     ISSN:  1944-9097     ISO Abbreviation:  J. Food Prot.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2011-01-11     Completed Date:  2011-01-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2281-4     Citation Subset:  IM    
Department of Genetics, Biology of Microorganisms, Anthropology, Evolution, University of Parma, Viale Usberti 11A, 43100, Parma, Italy.
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MeSH Terms
Cheese / microbiology*
Colony Count, Microbial
Consumer Product Safety
DNA, Bacterial / analysis
Enterotoxins / analysis,  genetics*
Escherichia coli / isolation & purification*
Food Contamination / analysis*
Food Microbiology
Gene Amplification
Milk / microbiology
Polymerase Chain Reaction*
Staphylococcus aureus / isolation & purification*
Reg. No./Substance:
0/DNA, Bacterial; 0/Enterotoxins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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