Document Detail

A polyoxyethylene-substituted bimetallic europium helicate for luminescent staining of living cells.
MedLine Citation:
PMID:  17879248     Owner:  NLM     Status:  MEDLINE    
The homoditopic ligand H2LC3 has been designed to form neutral triple-stranded bimetallic helicates of overall composition [Ln2(LC3)3]. The grafting of the polyoxyethylene fragments ensures water solubility and also favors cell penetration while being amenable to further derivatization. The ligand pKa values have been determined by spectrophotometric titration and range from 3.5 (sum of the first two) to 10.3. The thermodynamic stability of the helicates is large at physiological pH (logbeta23 in the range 22-23). The ligand triplet state has an adequate energy (0-phonon transition at approximately 20,800 cm(-1)) for sensitizing the luminescence of EuIII (Q=11%). Analysis of the EuIII emission spectrum points to an overall pseudo D3 symmetry for the metal environment. No significant effect of [Eu2(LC3)3] is observed on the viability of several cancerous cell lines (MCF-7, HeLa, Jurkat, and 5D10). The cell imaging properties of the EuIII helicate are demonstrated for the HeLa cell line by luminescence microscopy. Bright EuIII emission is seen for helicate concentration>50 microM and after 20-30 min loading time. The helicate stains the cytoplasm and the permeation mechanism is likely to be endocytosis.
Anne-Sophie Chauvin; Steve Comby; Bo Song; Caroline D B Vandevyver; Frédéric Thomas; Jean-Claude G Bünzli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chemistry (Weinheim an der Bergstrasse, Germany)     Volume:  13     ISSN:  0947-6539     ISO Abbreviation:  Chemistry     Publication Date:  2007  
Date Detail:
Created Date:  2007-11-22     Completed Date:  2008-02-26     Revised Date:  2009-08-04    
Medline Journal Info:
Nlm Unique ID:  9513783     Medline TA:  Chemistry     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  9515-26     Citation Subset:  IM    
Laboratory of Lanthanide Supramolecular Chemistry, Ecole Polytechnique Fédérale de Lausanne (EPFL), LCSL-BCH 1401, Switzerland.
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MeSH Terms
Cell Line
Cell Line, Tumor
Cell Survival / drug effects
Cells / chemistry*,  cytology
Europium / chemistry*,  toxicity
Molecular Structure
Polyethylene Glycols / chemistry*
Staining and Labeling / methods*
Water / chemistry
Reg. No./Substance:
0/Ligands; 0/Polyethylene Glycols; 0/Solutions; 7440-53-1/Europium; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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