Document Detail


A polyoxyethylene-substituted bimetallic europium helicate for luminescent staining of living cells.
MedLine Citation:
PMID:  17879248     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The homoditopic ligand H2LC3 has been designed to form neutral triple-stranded bimetallic helicates of overall composition [Ln2(LC3)3]. The grafting of the polyoxyethylene fragments ensures water solubility and also favors cell penetration while being amenable to further derivatization. The ligand pKa values have been determined by spectrophotometric titration and range from 3.5 (sum of the first two) to 10.3. The thermodynamic stability of the helicates is large at physiological pH (logbeta23 in the range 22-23). The ligand triplet state has an adequate energy (0-phonon transition at approximately 20,800 cm(-1)) for sensitizing the luminescence of EuIII (Q=11%). Analysis of the EuIII emission spectrum points to an overall pseudo D3 symmetry for the metal environment. No significant effect of [Eu2(LC3)3] is observed on the viability of several cancerous cell lines (MCF-7, HeLa, Jurkat, and 5D10). The cell imaging properties of the EuIII helicate are demonstrated for the HeLa cell line by luminescence microscopy. Bright EuIII emission is seen for helicate concentration>50 microM and after 20-30 min loading time. The helicate stains the cytoplasm and the permeation mechanism is likely to be endocytosis.
Authors:
Anne-Sophie Chauvin; Steve Comby; Bo Song; Caroline D B Vandevyver; Frédéric Thomas; Jean-Claude G Bünzli
Related Documents :
2004808 - Effect of urease on hela cell vacuolation induced by helicobacter pylori cytotoxin.
1826748 - Fastidious human adenovirus type 40 can propagate efficiently and produce plaques on a ...
12468638 - Seminal plasma activates cyclooxygenase-2 and prostaglandin e2 receptor expression and ...
21070888 - Cancer targeting potential of some ligand-anchored poly(propylene imine) dendrimers: a ...
24116948 - In vitro and in vivo antitumor effects of mpt0b014, a novel derivative aroylquinoline, ...
8271498 - Rapid filopodial sprouting induced by electrical stimulation in nerve terminals.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chemistry (Weinheim an der Bergstrasse, Germany)     Volume:  13     ISSN:  0947-6539     ISO Abbreviation:  Chemistry     Publication Date:  2007  
Date Detail:
Created Date:  2007-11-22     Completed Date:  2008-02-26     Revised Date:  2009-08-04    
Medline Journal Info:
Nlm Unique ID:  9513783     Medline TA:  Chemistry     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  9515-26     Citation Subset:  IM    
Affiliation:
Laboratory of Lanthanide Supramolecular Chemistry, Ecole Polytechnique Fédérale de Lausanne (EPFL), LCSL-BCH 1401, Switzerland. anne-sophie.chauvin@epfl.ch
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Line, Tumor
Cell Survival / drug effects
Cells / chemistry*,  cytology
Europium / chemistry*,  toxicity
Humans
Ligands
Luminescence*
Mice
Molecular Structure
Photochemistry
Polyethylene Glycols / chemistry*
Solutions
Spectrophotometry
Staining and Labeling / methods*
Water / chemistry
Chemical
Reg. No./Substance:
0/Ligands; 0/Polyethylene Glycols; 0/Solutions; 7440-53-1/Europium; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Enantioselective total synthesis of (+)-ottelione A, (-)-ottelione B, (+)-3-epi-ottelione A and prel...
Next Document:  Transition metal bisdithiolene complexes based on extended ligands with fused tetrathiafulvalene and...