| A polyketide macrolactone synthase from the filamentous fungus Gibberella zeae. | |
| | |
MedLine Citation:
|
PMID: 18427109 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Resorcylic acid lactones represent a unique class of fungal polyketides and display a wide range of biological activities, such as nanomolar inhibitors of Hsp90 and MAP kinase. The biosynthesis of these compounds is proposed to involve two fungal polyketide synthases (PKS) that function collaboratively to yield a 14-membered macrolactone with a resorcylate core. We report here the reconstitution of Gibberella zeae PKS13, which is the nonreducing PKS associated with zearalenone biosynthesis. Using a small molecule mimic of the natural hexaketide starter unit, we reconstituted the entire repertoire of PKS13 activities in vitro, including starter-unit selection, iterative condensation, regioselective C2-C7 cyclization, and macrolactone formation. PKS13 synthesized both natural 14-membered and previously uncharacterized 16-membered resorcylic acid lactones, indicating relaxed control in both iterative elongation and macrocyclization. PKS13 exhibited broad starter-unit specificities toward fatty acyl-CoAs ranging in sizes between C6 and C16 and displayed the highest activity toward decanoyl-CoA. PKS13 was shown to be active in Escherichia coli and synthesized numerous alkyl pyrones and alkyl resorcylic esters without exogenously supplied precursors. We demonstrated that PKS13 can interact with E. coli fatty acid biosynthetic machinery and can be primed with fatty-acyl ACPp at low-micromolar concentrations. PKS13 synthesized new polyketides in E. coli when the culture was supplemented with synthetic precursors, showcasing its utility in precursor-directed biosynthesis. PKS13 is therefore a highly versatile polyketide macrolactone synthase that is useful in the engineered biosynthesis of polyketides, including resorcylic acid lactones that are not found in nature. |
| | |
Authors:
|
Hui Zhou; Jixun Zhan; Kenji Watanabe; Xinkai Xie; Yi Tang |
Related Documents
:
|
21975559 - Post hoc analysis of a single iv infusion of zoledronic acid versus daily oral risedron... 18086849 - Antimycobacterial activity and mechanism of action of nas-91. 15774879 - Evidence that the essential response regulator yycf in streptococcus pneumoniae modulat... 22699569 - A new method for production of chiral 2-aryl-2-fluoropropanoic acids using an effective... 22493809 - Triterpenes from callicarpa integerrima champ. 22574939 - New abscisic acid-related metabolites from phellinus vaninii. 7365529 - Effects of dietary protein and efa deficiency on liver delta 5, delta 6 and delta 9 des... 2657479 - Selective depression of n-methyl-d-aspartate-mediated responses by dextrorphan in the h... 23107499 - Expression in lactococcus lactis of functional genes related to amino acid catabolism a... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-04-21 |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 105 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2008 Apr |
Date Detail:
|
Created Date: 2008-04-30 Completed Date: 2008-05-22 Revised Date: 2013-06-05 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
|
Languages: eng Pagination: 6249-54 Citation Subset: IM |
Affiliation:
|
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA 90095, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Escherichia coli
/
metabolism Gibberella / enzymology* Lactones / chemistry, metabolism* Polyketide Synthases / metabolism* Substrate Specificity |
| Grant Support | |
ID/Acronym/Agency:
|
1R21GM077264-01A1/GM/NIGMS NIH HHS; R01 GM085128-01/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Lactones; 79956-01-7/Polyketide Synthases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The molecular and cellular basis of olfactory-driven behavior in Anopheles gambiae larvae.
Next Document: Antiinflammatory cAMP signaling and cell migration genes co-opted by the anthrax bacillus.