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The polyacetylenes falcarinol and falcarindiol affect stress responses in myotube cultures in a biphasic manner.
MedLine Citation:
PMID:  18846258     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The effects of the bioactive polyacetylenes, falcarinol and falcarindiol, present in carrots, celery, celeriac and other umbelliferous vegetables, on the stress responses in primary myotube cultures, were studied. Biphasic responses on cellular stress responses in myotube cultures were investigated by exposing them to various concentrations of falcarinol and falcarindiol for 24 h before testing effects of 100 microM H(2)O(2) on the intracellular formation of reactive oxygen species (ROS), transcription of the antioxidative enzyme cytosolic glutathione peroxidase (cGPx), and the heat shock proteins (HSP) HSP70 and HO1. At low concentrations (1.6 to 25 microM) polyacetylenes caused a slightly accelerated intra-cellular ROS formation, increased cGPx transcription and decreased HSP70 and HO1 transcription. The increased cGPx transcription may be interpreted as an adaptive response to the increased ROS formation and may have caused a reduced demand for the protective functions of the HSPs. ROS formation, however, was substantially decreased after pre-incubation with both polyacetylenes at 50 and 100 microM, the cGPx transcription was reduced and the HSP70 and HO1 transcription increased, indicating a need for the protective and repairing functions of the HSPs. In conclusion, pre-incubation with low concentrations of both polyacetylenes prior to H(2)O(2) exposure induced a cytoprotective effect whereas higher concentrations had adverse effects.
Authors:
Jette F Young; Lars P Christensen; Peter K Theil; Niels Oksbjerg
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Publication Detail:
Type:  Journal Article     Date:  2008-08-08
Journal Detail:
Title:  Dose-response : a publication of International Hormesis Society     Volume:  6     ISSN:  1559-3258     ISO Abbreviation:  Dose Response     Publication Date:  2008  
Date Detail:
Created Date:  2008-10-10     Completed Date:  2010-06-09     Revised Date:  2013-05-23    
Medline Journal Info:
Nlm Unique ID:  101308899     Medline TA:  Dose Response     Country:  United States    
Other Details:
Languages:  eng     Pagination:  239-51     Citation Subset:  -    
Affiliation:
Department of Food Science, Faculty of Agricultural Sciences, University of Aarhus, Blichers Allé, Tjele, Denmark. JetteF.Young@agrsci.dk
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