| A poliovirus hybrid expressing a neutralization epitope from the major outer membrane protein of Chlamydia trachomatis is highly immunogenic. | |
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MedLine Citation:
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PMID: 7691749 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Trachoma and sexually transmitted diseases caused by Chlamydia trachomatis are major health problems worldwide. Epitopes on the major outer membrane protein (MOMP) of C. trachomatis have been identified as important targets for the development of vaccines. In order to examine the immunogenicity of a recombinant vector expressing a chlamydial epitope, a poliovirus hybrid was constructed in which part of neutralization antigenic site I of poliovirus type 1 Mahoney (PV1-M) was replaced by a sequence from variable domain I of the MOMP of C. trachomatis serovar A. The chlamydial sequence included the neutralization epitope VAGLEK. This hybrid was viable, grew very well compared with PV1-M, and expressed both poliovirus and chlamydial antigenic determinants. When inoculated into rabbits, this hybrid was highly immunogenic, inducing a strong response against both PV1-M and C. trachomatis serovar A. Antichlamydia titers were 10- to 100-fold higher than the titers induced by equimolar amounts of either purified MOMP or a synthetic peptide expressing the VAGLEK epitope. Furthermore, rabbit antisera raised against this hybrid neutralized chlamydial infectivity both in vitro, for hamster kidney cells, and passively in vivo, for conjunctival epithelia of cynomolgus monkeys. Because poliovirus infection induces a strong mucosal immune response in primates and humans, these results indicate that poliovirus-chlamydia hybrids could become powerful tools for the study of mucosal immunity to chlamydial infection and for the development of recombinant chlamydial vaccines. |
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Authors:
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A D Murdin; H Su; D S Manning; M H Klein; M J Parnell; H D Caldwell |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Infection and immunity Volume: 61 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 1993 Oct |
Date Detail:
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Created Date: 1993-11-16 Completed Date: 1993-11-16 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4406-14 Citation Subset: IM |
Affiliation:
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Connaught Centre for Biotechnology Research, Willowdale, Ontario, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antibodies, Bacterial / immunology Antigens, Bacterial / genetics Bacterial Outer Membrane Proteins / immunology* Chlamydia trachomatis / immunology* Conjunctivitis, Inclusion / immunology, prevention & control Epitopes Immunization, Passive Macaca fascicularis Molecular Sequence Data Mucous Membrane / immunology Poliovirus / genetics Recombinant Proteins / immunology Vaccines, Synthetic |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/Bacterial Outer Membrane Proteins; 0/Epitopes; 0/Recombinant Proteins; 0/Vaccines, Synthetic |
| Comments/Corrections | |
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