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The platelet-rich plasma clot: a standardized in-vitro clot formation protocol for investigations of sonothrombolysis under physiological flows.
MedLine Citation:
PMID:  21519235     Owner:  NLM     Status:  Publisher    
No agreement exists about which protocol for in-vitro clot formation is suitable for sonothrombolysis investigations. Lysis rates vary considerably because of different clotting processes and cannot be compared. We aim to establish a new protocol for in-vitro coagulation to permit standardized sonothrombolysis investigations. The proposed procedure is based upon clots prepared from platelet-rich plasma (PRP). This clot material (group A) was compared with the two most commonly used procedures, namely, recalcification of citrate-anticoagulated whole venous blood (group B) and spontaneous clotting of nonanticoagulated venous blood (group C). Histological examinations were performed and clot stability was tested under physiological flow conditions in vitro for all groups (each n = 10). Lysis rates measured by mass loss were compared using buffered plasma and recombinant tissue plasminogen activator (60 kU/ml), or buffered plasma alone. PRP clots displayed a high degree of similarity to emboli specimens in histological examinations and remained stable under pulsatile flow conditions. B and C clots were mechanically unstable and did not resist physiological flow and pressure. Measuring the lysis rate by weighing seems to be inaccurate, with lowest variability in PRP clots. PRP clots appeared more resistant to lysis. PRP clots should be used for standardized sonothrombolysis investigations.
Florian C Roessler; Marcus Ohlrich; Jan H Marxsen; Florian Stellmacher; Andreas Sprenger; Carl-Erik Dempfle; Günter Seidel
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-23
Journal Detail:
Title:  Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis     Volume:  -     ISSN:  1473-5733     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9102551     Medline TA:  Blood Coagul Fibrinolysis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
aDepartment of Neurology, University Lübeck, Ratzeburger Allee, Lübeck, Germany bDepartment of Internal Medicine, Haematology, University Lübeck, Ratzeburger Allee, Lübeck, Germany cResearch Center Borstel, Clinical and Experimental Pathology, Parkallee, Borstel, Germany dDepartment of Internal Medicine, University Medical Center Mannheim, Theodor Kutzer Ufer, Mannheim, Germany eDepartment of Neurology, Asklepios Clinic Heidberg, Tangstedter Landstraße, Hamburg, Germany.
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