| A plant-specific calreticulin is a key retention factor for a defective brassinosteroid receptor in the endoplasmic reticulum. | |
| | |
MedLine Citation:
|
PMID: 19597144 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Mammalian calreticulin (CRT) is a multifunctional Ca(2+)-binding protein involved in more than 40 cellular processes in various subcellular compartments, such as Ca(2+) storage and protein folding in the endoplasmic reticulum (ER). CRT homologues were discovered in plants almost 15 years ago, and recent studies revealed that many plant species contain 2 or more CRTs that are members of 2 distinct families, the CRT1/2 family and the plant-specific CRT3 family. However, little is known about their physiological functions. Here we report ebs2 (EMS-mutagenized bri1 suppressor 2) as an allele-specific suppressor of bri1-9, a dwarf Arabidopsis mutant caused by retention of a defective brassinosteroid receptor in the ER. EBS2 encodes the Arabidopsis CRT3 that interacts with ER-localized bri1-9 in a glycan-dependent manner. Loss-of-function ebs2 mutations compromise ER retention of bri1-9 and suppress its dwarfism, whereas EBS2 over-expression enhances its dwarf phenotype. In contrast, mutations of 2 other CRTs or their membrane-localized homologues calnexins had little effect on bri1-9. A domain-swapping experiment revealed that the positively charged C-terminal tail of CRT3 is crucial for its "bri1-9-retainer" function. Our study revealed not only a functional role for a plant-specific CRT, but also functional diversity among the 3 Arabidopsis CRT paralogues. |
| | |
Authors:
|
Hua Jin; Zhi Hong; Wei Su; Jianming Li |
Related Documents
:
|
19516994 - Phospholipase dzeta2 drives vesicular secretion of auxin for its polar cell-cell transp... 11255514 - Pleiotropic morphological and abiotic stress resistance phenotypes of the hyper-abscisi... 10982454 - Zinc deficiency up-regulates expression of high-affinity phosphate transporter genes in... 19457984 - Characterization of arabidopsis 6-phosphogluconolactonase t-dna insertion mutants revea... 11539044 - Effects of co2 and photosynthetic photon flux on yield, gas exchange and growth rate of... 21625984 - An ecological genomic approach challenging the paradigm of differential plant responses... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-07-13 |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 106 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2009 Aug |
Date Detail:
|
Created Date: 2009-08-26 Completed Date: 2009-09-21 Revised Date: 2011-09-26 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
|
Languages: eng Pagination: 13612-7 Citation Subset: IM |
Affiliation:
|
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109-1048, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alleles Amino Acid Sequence Arabidopsis / genetics, metabolism* Arabidopsis Proteins / chemistry, genetics, metabolism* Calreticulin / chemistry, genetics, metabolism* Cholestanols / metabolism* Cloning, Molecular Endoplasmic Reticulum / metabolism* Gene Expression Regulation, Plant Genes, Plant Molecular Sequence Data Mutation / genetics Phenotype Plant Lectins / metabolism Protein Binding Receptors, Cell Surface / metabolism* Species Specificity Steroids, Heterocyclic / metabolism* Suppression, Genetic |
| Grant Support | |
ID/Acronym/Agency:
|
R01 GM060519-08/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Arabidopsis Proteins; 0/Calreticulin; 0/Cholestanols; 0/Plant Lectins; 0/Receptors, Cell Surface; 0/Steroids, Heterocyclic; 72962-43-7/brassinolide |
| Comments/Corrections | |
Comment In:
|
Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13151-2
[PMID:
19666569
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Modeling oxygen transport in surgical tissue transfer.
Next Document: Wild birds of declining European species are dying from a thiamine deficiency syndrome.