| A pilot study of sampling subcutaneous adipose tissue to examine biomarkers of cancer risk. | |
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MedLine Citation:
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PMID: 19139016 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Examination of adipose tissue biology may provide important insight into mechanistic links for the observed association between higher body fat and risk of several types of cancer, in particular colorectal and breast cancer. We tested two different methods of obtaining adipose tissue from healthy individuals. Ten overweight or obese (body mass index, 25-40 kg/m(2)), postmenopausal women were recruited. Two subcutaneous abdominal adipose tissue samples were obtained per individual (i.e., right and left lower abdominal regions) using two distinct methods (method A: 14-gauge needle with incision, versus method B: 16-gauge needle without incision). Gene expression was examined at the mRNA level for leptin, adiponectin, aromatase, interleukin 6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) in flash-frozen tissue, and at the protein level for leptin, adiponectin, IL-6, and TNF-alpha following short-term culture. Participants preferred biopsy method A and few participants reported any of the usual minor side effects. Gene expression was detectable for leptin, adiponectin, and aromatase, but was below detectable limits for IL-6 and TNF-alpha. For detectable genes, relative gene expression in adipose tissue obtained by methods A and B was similar for adiponectin (r = 0.64, P = 0.06) and leptin (r = 0.80, P = 0.01), but not for aromatase (r = 0.37,P = 0.34). Protein levels in tissue culture supernatant exhibited good intra-assay agreement [coefficient of variation (CV), 1-10%], with less agreement for intraindividual agreement (CV, 17-29%) and reproducibility, following one freeze-thaw cycle (CV, >14%). Subcutaneous adipose tissue biopsies from healthy, overweight individuals provide adequate amounts for RNA extraction, gene expression, and other assays of relevance to cancer prevention research. |
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Authors:
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Kristin L Campbell; Karen W Makar; Mario Kratz; Karen E Foster-Schubert; Anne McTiernan; Cornelia M Ulrich |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cancer prevention research (Philadelphia, Pa.) Volume: 2 ISSN: 1940-6215 ISO Abbreviation: Cancer Prev Res (Phila) Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2009-01-13 Completed Date: 2009-03-18 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 101479409 Medline TA: Cancer Prev Res (Phila) Country: United States |
Other Details:
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Languages: eng Pagination: 37-42 Citation Subset: IM |
Affiliation:
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Cancer Prevention Program, Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M4-B402, Seattle, WA 98109-1024, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adiponectin
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biosynthesis,
genetics Aromatase / biosynthesis, genetics Biopsy / methods Breast Neoplasms / genetics*, pathology Colorectal Neoplasms / genetics*, pathology Female Gene Expression Humans Interleukin-6 / biosynthesis, genetics Leptin / biosynthesis, genetics Obesity / complications*, genetics, pathology Overweight / complications, genetics, pathology Pilot Projects RNA, Messenger / analysis Risk Factors Subcutaneous Fat / metabolism, pathology*, surgery Tumor Markers, Biological / analysis*, genetics Tumor Necrosis Factor-alpha / biosynthesis, genetics |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA 77572-01/CA/NCI NIH HHS; R01 CA077572-01A1/CA/NCI NIH HHS; U54 CA116847/CA/NCI NIH HHS; U54 CA116847-040004/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Adiponectin; 0/Interleukin-6; 0/Leptin; 0/RNA, Messenger; 0/Tumor Markers, Biological; 0/Tumor Necrosis Factor-alpha; EC 1.14.14.1/Aromatase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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