| A pilot study of gas chromatograph/mass spectrometry-based serum metabolic profiling of colorectal cancer after operation. | |
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MedLine Citation:
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PMID: 19340605 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Colorectal cancer (CRC) is mainly depended on the radical operation, the changing energy metabolism after operation reflects the extent, the magnitude, and the degree of surgical trauma. The aim of this study was to analyse the biochemical perturbation in the serum of CRC after operation and to evaluate their involvement in the progression of CRC. Gas chromatograph-mass spectrometry (GC-MS) in combination with pattern recognition techniques (Partial least squares discriminant analysis, supervised clustering analysis) was used to analyze serum metabolome in 30 CRC patients. A 34 endogenous metabolites included amino acid, fatty acid, carbohydrate and other intermediate metabolites were identified. Partial least squares discriminant analysis based on these metabolites discriminated preoperative from postoperative CRC group. Compared with preoperative CRC patients group, decreases in L-valine, 5-oxo-L-proline, 1-Deoxyglucose, D-turanose, D-maltose, arachidonic acid and hexadecanoic acid levels and increases in L-tyrosine levels were observed in postoperative CRC patients group. The result demonstrated the GC-MS technique is an valuable tool for the characterization of the metabolic perturbation, and the metabolomic study will certainly benefit for monitoring the nutrition state of CRC patients, the prognosis and therapy evaluation of CRC patients after operation. |
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Authors:
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Yanlei Ma; Weijie Liu; Jiayuan Peng; Long Huang; Peng Zhang; Xiaoping Zhao; Yiyu Cheng; Huanlong Qin |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-02 |
Journal Detail:
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Title: Molecular biology reports Volume: 37 ISSN: 1573-4978 ISO Abbreviation: Mol. Biol. Rep. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-03 Completed Date: 2010-05-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0403234 Medline TA: Mol Biol Rep Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1403-11 Citation Subset: IM |
Affiliation:
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Department of Surgery, The Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aged Blood Chemical Analysis / methods* China Cluster Analysis Colorectal Neoplasms / metabolism*, surgery Discriminant Analysis Female Gas Chromatography-Mass Spectrometry / methods* Humans Male Metabolome / physiology* Middle Aged Postoperative Period |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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