Document Detail


A physical and genetic map of the Stigmatella aurantiaca DW4/3.1 chromosome.
MedLine Citation:
PMID:  7934859     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A physical map of the myxobacterium Stigmatella aurantiaca DW4/3.1 chromosome was constructed by pulsed-field gel (PFG) long-range mapping. One-and two-dimensional pulsed-field gel analyses were used together with reciprocal double-restriction, cross-hybridization and hybridization fingerprint analysis. These PFG results were confirmed by Smith-Birnstiel analysis, by Southern hybridization using linking clones and clones of a lambda genomic library for the determination of adjacent restriction fragments and by transposon insertion mapping using defined genomic sequences for hybridization. It was thus possible to construct a circular restriction map of the single 9.35 Mbp chromosome of S. aurantiaca based on the endonucleases Asel and Spel. Genetic loci as well as the replication origin were located on the physical map by Southern hybridization using heterologous (derived from Myxococcus xanthus, Escherichia coli and Streptomyces lividans) and homologous probes that are mainly involved in development and cell motility.
Authors:
B Neumann; A Pospiech; H U Schairer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular microbiology     Volume:  10     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  1993 Dec 
Date Detail:
Created Date:  1994-11-23     Completed Date:  1994-11-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1087-99     Citation Subset:  IM    
Affiliation:
Zentrum für Molekulare Biologie (ZMBH), Universität Heidelberg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Bacteriophage lambda / genetics
Chromosome Mapping*
Chromosomes, Bacterial*
Cloning, Molecular
DNA Probes
DNA, Bacterial / genetics,  isolation & purification
Electrophoresis, Gel, Pulsed-Field
Gene Library
Genetic Vectors
Genome, Bacterial*
Myxococcales / genetics*
Nucleic Acid Hybridization
Restriction Mapping
Chemical
Reg. No./Substance:
0/DNA Probes; 0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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