Document Detail


The phycocyanin-associated rod linker proteins of the phycobilisome of Gloeobacter violaceus PCC 7421 contain unusually located rod-capping domains.
MedLine Citation:
PMID:  16617515     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Gloeobacter violaceus PCC 7421 is a unique cyanobacterium that has no thylakoids and whose genome has been sequenced [Y. Nakamura, T. Kaneko, S. Sato, M. Mimuro, H. Miyashita, T. Tsuchiya, S. Sasamoto, A. Watanabe, K. Kawashima, Y. Kishida, C. Kiyokawa, M. Kohara, M. Matsumoto, A. Matsuno, N. Nakazaki, S. Shimpo, C. Takeuchi, M. Yamada, S. Tabata, Complete Genome Structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids. DNA Research 10 (2003) 137-145]. Phycobilisomes of G. violaceus were isolated and analyzed by SDS-PAGE followed by N-terminal sequencing. Three rod-linker subunits (CpeC, CpeD and CpeE) were identified as predicted from the genome sequence. The cpcC1 and cpcC2 genes at order locus named (OLN) glr0950 and gll 3219 encoding phycocyanin-associated linker proteins from G. violaceus are 56 and 55 amino acids longer at the N-terminus than the open reading frame proposed in the genome. The two amino acid extensions showed a 66% identity to one another. Also, the N-terminal extensions of these sequences were similar to domains in both the rod-capping-linker protein CpcD2 and to the C-terminus domain of the phycoerythrin-associated linker protein CpeC. These domains are not only unusual in their N-terminal location, but are unusual in that they are more closely related in sequence similarity to the C-terminus domain of the phycoerythrin-associated linker, CpeC of G. violaceus, than to the C-terminus domain of phycocyanin-associated linker CpcC in other cyanobacteria. These linker proteins with unique special domains are indicators of the unusual structure of the phycobilisomes of G. violaceus.
Authors:
Emma Berta Gutiérrez-Cirlos; Bertha Pérez-Gómez; David W Krogmann; Carlos Gómez-Lojero
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1757     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-04-14     Completed Date:  2006-04-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  130-4     Citation Subset:  IM    
Affiliation:
Unidad de Biomedicina FES-Iztacala UNAM Av. De los Barrios 1, Los Reyes Iztacala, Tlalnepantla, Edo de México 54090, México.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Bacterial Proteins / genetics*
Cyanobacteria / metabolism*
Light-Harvesting Protein Complexes / genetics*
Molecular Sequence Data
Phycobilisomes / chemistry*
Phycocyanin / metabolism*
Phycoerythrin / metabolism
Protein Structure, Tertiary
Sequence Alignment
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Light-Harvesting Protein Complexes; 0/Phycobilisomes; 0/cpcD phycobilisome linker protein, cyanobacteria; 11016-15-2/Phycocyanin; 11016-17-4/Phycoerythrin

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