| A photocrosslinking assay for reporting protein interactions in polyketide and fatty acid synthases. | |
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MedLine Citation:
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PMID: 21918773 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Understanding protein-protein interactions that occur between ACP and KS domains of polyketide synthases and fatty acid synthases is critical to improving the scope and efficiency of combinatorial biosynthesis efforts aimed at producing non-natural polyketides. Here, we report a facile strategy for rapidly reporting such ACP-KS interactions based on the incorporation of an amino acid with photocrosslinking functionality. Crucially, this photocrosslinking strategy can be applied to any polyketide or fatty acid synthase regardless of substrate specificity, and can be adapted to a high-throughput format for directed evolution studies. |
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Authors:
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Zhixia Ye; Morgan Bair; Hemant Desai; Gavin J Williams |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-14 |
Journal Detail:
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Title: Molecular bioSystems Volume: - ISSN: 1742-2051 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101251620 Medline TA: Mol Biosyst Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Chemistry, North Carolina State University, Raleigh, NC, 27695 USA. gavin_williams@ncsu.edu. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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