| The phosphorylation state of the retinoblastoma (RB) protein in G0/G1 is dependent on growth status. | |
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MedLine Citation:
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PMID: 2000225 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The product of the retinoblastoma gene (RB) is a nuclear phosphoprotein which is thought to regulate the proliferation of cells. Its phosphorylation state changes with passage through the cell cycle and it has been proposed that RB protein in its hypo-phosphorylated form prevents cells proliferating. We have investigated the phosphorylation state of the RB protein in an actively-dividing human B-lymphoblastoid cell line and after cell cycle arrest caused by alpha-Interferon (alpha-IFN). We show that the phosphorylation state of the RB protein in cells with 2N DNA content depends on whether the cells are actively cycling. Our data is compatible with the proposal that dephosphorylation of the RB protein allows cells to enter a quiescent state. This study sheds light on the molecular mechanisms which may mediate the cytostatic effects of alpha-IFN. |
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Authors:
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N S Thomas; L C Burke; A Bybee; D C Linch |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Oncogene Volume: 6 ISSN: 0950-9232 ISO Abbreviation: Oncogene Publication Date: 1991 Feb |
Date Detail:
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Created Date: 1991-04-09 Completed Date: 1991-04-09 Revised Date: 2009-09-29 |
Medline Journal Info:
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Nlm Unique ID: 8711562 Medline TA: Oncogene Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 317-22 Citation Subset: IM |
Affiliation:
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Department of Haematology, University College and Middlesex School of Medicine, London, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line DNA, Neoplasm / analysis G0 Phase* / drug effects G1 Phase* / drug effects Humans Interferon Type I / pharmacology Phosphorylation Retinoblastoma Protein / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 0/Interferon Type I; 0/Retinoblastoma Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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