Document Detail


The phosphorylation of high mobility group proteins 14 and 17 and their distribution in chromatin.
MedLine Citation:
PMID:  6461658     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The phosphorylation of the high mobility group (HMG) proteins has been investigated in mouse Ehrlich ascites, L1210 and P388 leukemia cells, human colon carcinoma cells (HT-29), and Chinese hamster ovary cells. HMG 14 and 17, but not HMB 1 and 2, were phosphorylated in the nuclei of all cell lines with a serine being the site of modification for both proteins in Ehrlich ascites cells. Phosphorylation of HMG 14 and 17 was greatly reduced in cultured cells at plateau phase in comparison to log phase cells, suggesting that modification of HMG 14 and 17 is growth-associated. However, phosphorylation was not linked to DNA synthesis, since incorporation of 32P did not vary through G1 and S phase in synchronized Chinese hamster ovary cells. Treatment of HT-29 or Ehrlich ascites cells with sodium butyrate reduced HMG phosphorylation by 30 and 70%, respectively. The distribution of the phosphorylated HMG proteins in chromatin was examined using micrococcal nuclease and DNase I. 32P-HMG 14 and 17 were preferentially associated with micrococcal nuclease-sensitive regions as demonstrated by the release of a substantial fraction of the phosphorylated forms of these proteins under conditions which solubilized less than 3% of the DNA. Short digestions with DNase I did not show a marked release of 32P-HMG 14 or 17.
Authors:
J D Saffer; R I Glazer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  257     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1982 Apr 
Date Detail:
Created Date:  1982-06-24     Completed Date:  1982-06-24     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4655-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma, Ehrlich Tumor / metabolism
Cell Line
Chromatin / metabolism*
Chromosomal Proteins, Non-Histone / metabolism*
Colonic Neoplasms / metabolism
Cricetinae
Cricetulus
Female
High Mobility Group Proteins
Histones / metabolism
Humans
Kinetics
Leukemia L1210 / metabolism
Leukemia P388 / metabolism
Mice
Ovary
Phosphorylation
Chemical
Reg. No./Substance:
0/Chromatin; 0/Chromosomal Proteins, Non-Histone; 0/High Mobility Group Proteins; 0/Histones

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