Document Detail

The phosphonomethyl analogue of 3-phosphoglycerate is a potent competitive inhibitor of phosphoglycerate mutases.
MedLine Citation:
PMID:  2996505     Owner:  NLM     Status:  MEDLINE    
The phosphonomethyl analogue of 3-phosphoglycerate (2-hydroxy-4-phosphonobutanoate) is a potent competitive inhibitor of cofactor-dependent phosphoglycerate mutase from yeast and of cofactor-independent phosphoglycerate mutase from wheat germ. For the yeast enzyme Ki is 1.3 mM (Km for substrate is 0.71 mM); for the wheatgerm enzyme Ki is 18 mM (Km for substrate is 0.86 mM). This analogue should be a useful tool for n.m.r. spectroscopic studies on the mechanism of action of the two mutases. The arsonomethyl analogue of 3-phosphoglycerate (4-arsono-2-hydroxybutanoate) was a relatively poor inhibitor.
S M McAleese; L A Fothergill-Gilmore; H B Dixon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  230     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1985 Sep 
Date Detail:
Created Date:  1985-11-21     Completed Date:  1985-11-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  535-42     Citation Subset:  IM    
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MeSH Terms
Binding Sites
Glyceric Acids / pharmacology
Hydroxybutyrates / pharmacology*
Models, Chemical
Phosphoglycerate Mutase / antagonists & inhibitors*
Phosphotransferases / antagonists & inhibitors*
Reg. No./Substance:
0/Glyceric Acids; 0/Hydroxybutyrates; 51360-19-1/2-hydroxy-4-phosphonobutyric acid; 820-11-1/3-phosphoglycerate; EC 2.7.-/Phosphotransferases; EC Mutase

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