Document Detail

The phenylalanine ammonia-lyase gene family in raspberry. Structure, expression, and evolution.
MedLine Citation:
PMID:  11553751     Owner:  NLM     Status:  MEDLINE    
In raspberry (Rubus idaeus), development of fruit color and flavor are critically dependent on products of the phenylpropanoid pathway. To determine how these metabolic functions are integrated with the fruit ripening program, we are examining the properties and expression of key genes in the pathway. Here, we report that L- phenylalanine ammonia-lyase (PAL) is encoded in raspberry by a family of two genes (RiPAL1 and RiPAL2). RiPAL1 shares 88% amino acid sequence similarity to RiPAL2, but phylogenetic analysis places RiPAL1 and RiPAL2 in different clusters within the plant PAL gene family. The spatial and temporal expression patterns of the two genes were investigated in various vegetative and floral tissues using the reverse transcriptase competitor polymerase chain reaction assay. Although expression of both genes was detected in all tissues examined, RiPAL1 was associated with early fruit ripening events, whereas expression of RiPAL2 correlated more with later stages of flower and fruit development. Determination of the absolute levels of the two transcripts in various tissues showed that RiPAL1 transcripts were 3- to 10-fold more abundant than those of RiPAL2 in leaves, shoots, roots, young fruits, and ripe fruits. The two RiPAL genes therefore appear to be controlled by different regulatory mechanisms.
A Kumar; B E Ellis
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant physiology     Volume:  127     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-09-12     Completed Date:  2001-12-04     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  230-9     Citation Subset:  IM    
The Biotechnology Laboratory and Faculty of Agricultural Sciences, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4.
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MeSH Terms
Acyltransferases / genetics,  metabolism
Cloning, Molecular
Coenzyme A Ligases / genetics*,  metabolism
DNA, Complementary
DNA, Plant
Evolution, Molecular
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Multigene Family
Phenylalanine Ammonia-Lyase / classification,  genetics*
Plant Structures / genetics,  metabolism
Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Rosales / genetics*,  metabolism
Reg. No./Substance:
0/DNA, Complementary; 0/DNA, Plant; EC 2.3.-/Acyltransferases; EC synthetase; EC Ammonia-Lyase; EC 6.2.1.-/Coenzyme A Ligases; EC ligase

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