| The phantom burster model for pancreatic beta-cells. | |
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MedLine Citation:
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PMID: 11106596 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pancreatic beta-cells exhibit bursting oscillations with a wide range of periods. Whereas periods in isolated cells are generally either a few seconds or a few minutes, in intact islets of Langerhans they are intermediate (10-60 s). We develop a mathematical model for beta-cell electrical activity capable of generating this wide range of bursting oscillations. Unlike previous models, bursting is driven by the interaction of two slow processes, one with a relatively small time constant (1-5 s) and the other with a much larger time constant (1-2 min). Bursting on the intermediate time scale is generated without need for a slow process having an intermediate time constant, hence phantom bursting. The model suggests that isolated cells exhibiting a fast pattern may nonetheless possess slower processes that can be brought out by injecting suitable exogenous currents. Guided by this, we devise an experimental protocol using the dynamic clamp technique that reliably elicits islet-like, medium period oscillations from isolated cells. Finally, we show that strong electrical coupling between a fast burster and a slow burster can produce synchronized medium bursting, suggesting that islets may be composed of cells that are intrinsically either fast or slow, with few or none that are intrinsically medium. |
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Authors:
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R Bertram; J Previte; A Sherman; T A Kinard; L S Satin |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biophysical journal Volume: 79 ISSN: 0006-3495 ISO Abbreviation: Biophys. J. Publication Date: 2000 Dec |
Date Detail:
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Created Date: 2001-01-18 Completed Date: 2001-01-18 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2880-92 Citation Subset: IM |
Affiliation:
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School of Science, Pennsylvania State University, Erie, Pennsylvania 16563, USA. bertram@sb.fsu.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Electric Conductivity Islets of Langerhans / cytology, physiology* Kinetics Membrane Potentials Mice Models, Biological* Models, Theoretical Patch-Clamp Techniques |
| Grant Support | |
ID/Acronym/Agency:
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R01DK46409/DK/NIDDK NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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