Document Detail

A phage display system with unnatural amino acids.
MedLine Citation:
PMID:  15584720     Owner:  NLM     Status:  MEDLINE    
We report a general method to display peptide-containing unnatural amino acids on filamentous M13 phage. Five distinct unnatural amino acids were site-specifically incorporated at the N-terminal of the M13 phage minor coat protein pIII. Phages that contain p-azidophenylalanine can undergo a highly specific azide-alkyne [3 + 2] cycloaddition reaction with an alkyne-derivatized fluorophore. The generalization of phage display to include unnatural amino acids should significantly increase the scope of phage display technology.
Feng Tian; Meng-Lin Tsao; Peter G Schultz
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  126     ISSN:  0002-7863     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-12-08     Completed Date:  2005-02-15     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  15962-3     Citation Subset:  IM    
Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
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MeSH Terms
Amino Acids / genetics*,  metabolism*
Capsid Proteins
Carrier Proteins / genetics,  metabolism
DNA-Binding Proteins / genetics,  metabolism
Methanococcus / genetics,  metabolism
Peptide Biosynthesis / genetics*
Peptide Library*
Viral Fusion Proteins / genetics,  metabolism
Grant Support
Reg. No./Substance:
0/Amino Acids; 0/Capsid Proteins; 0/Carrier Proteins; 0/DNA-Binding Proteins; 0/Peptide Library; 0/Viral Fusion Proteins; 0/streptavidin-binding peptide

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