| Persistence of antibiotic resistance: evaluation of a probiotic approach using antibiotic-sensitive Megasphaera elsdenii strains to prevent colonization of swine by antibiotic-resistant strains. | |
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MedLine Citation:
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PMID: 21821757 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Megasphaera elsdenii is a lactate-fermenting, obligately anaerobic bacterium commonly present in the gastrointestinal tracts of mammals, including humans. Swine M. elsdenii strains were previously shown to have high levels of tetracycline resistance (MIC=64 to >256 μg/ml) and to carry mosaic (recombinant) tetracycline resistance genes. Baby pigs inherit intestinal microbiota from the mother sow. In these investigations we addressed two questions. When do M. elsdenii strains from the sow colonize baby pigs? Can five antibiotic-sensitive M. elsdenii strains administered intragastrically to newborn pigs affect natural colonization of the piglets by antibiotic-resistant (AR) M. elsdenii strains from the mother? M. elsdenii natural colonization of newborn pigs was undetectable (<10(4) CFU/g [wet weight] of feces) prior to weaning (20 days after birth). After weaning, all pigs became colonized (4 × 10(5) to 2 × 10(8) CFU/g feces). In a separate study, 61% (76/125) of M. elsdenii isolates from a gravid sow never exposed to antibiotics were resistant to chlortetracycline, ampicillin, or tylosin. The inoculation of the sow's offspring with mixtures of M. elsdenii antibiotic-sensitive strains prevented colonization of the offspring by maternal AR strains until at least 11 days postweaning. At 25 and 53 days postweaning, however, AR strains predominated. Antibiotic susceptibility phenotypes and single nucleotide polymorphism (SNP)-based identities of M. elsdenii isolated from sow and offspring were unexpectedly diverse. These results suggest that dosing newborn piglets with M. elsdenii antibiotic-sensitive strains delays but does not prevent colonization by maternal resistant strains. M. elsdenii subspecies diversity offers an explanation for the persistence of resistant strains in the absence of antibiotic selection. |
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Authors:
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Thad B Stanton; Samuel B Humphrey |
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Publication Detail:
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Type: Journal Article Date: 2011-08-05 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 77 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-06 Completed Date: 2012-02-02 Revised Date: 2013-05-23 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 7158-66 Citation Subset: IM |
Affiliation:
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Food Safety and Enteric Pathogens Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, IA 50010, USA. Thad.Stanton@ars.usda.gov |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/JF297584; JF297585 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Anti-Bacterial Agents / pharmacology Antibiosis* Bacterial Load Base Sequence DNA, Bacterial / chemistry, genetics Drug Resistance, Bacterial* Feces / microbiology Gastrointestinal Tract / microbiology* Megasphaera / drug effects, growth & development*, isolation & purification Molecular Sequence Data Probiotics / administration & dosage* Sequence Analysis, DNA Swine |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/DNA, Bacterial |
| Comments/Corrections | |
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