Document Detail


The permease gene nagE2 is the key to N-acetylglucosamine sensing and utilization in Streptomyces coelicolor and is subject to multi-level control.
MedLine Citation:
PMID:  20487300     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The availability of nutrients is a major determinant for the timing of morphogenesis and antibiotic production in the soil-dwelling bacterium Streptomyces coelicolor. Here we show that N-acetylglucosamine transport, the first step of an important nutrient signalling cascade, is mediated by the NagE2 permease of the phosphotransferase system, and that the activity of this permease is linked to nutritional control of development and antibiotic production. The permease serves as a high-affinity transporter for N-acetylglucosamine (K(m) of 2.6 microM). The permease complex was reconstituted with individually purified components. This showed that uptake of N-acetylglucosamine requires a phosphoryl group transfer from phosphoenolpyruvate via the phosphotransferases EI, HPr and IIA(Crr) to NagF, which in turn phosphorylates N-acetylglucosamine during transport. Transcription of the nagF and nagE2 genes is induced by N-acetylglucosamine. Nutrient signalling by N-acetylglucosamine that triggers the onset of development was abolished in the nagE2 and nagF mutants. nagE2 is subject to multi-level control by the global transcription factor DasR and the activator AtrA that also stimulates genes for antibiotic actinorhodin biosynthesis. Hence, it is apparent that streptomycetes tightly control the nutritional state in a complex manner to ensure the correct timing for the developmental programme.
Authors:
Harald Nothaft; Sébastien Rigali; Bart Boomsma; Magdalena Swiatek; Kenneth J McDowall; Gilles P van Wezel; Fritz Titgemeyer
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular microbiology     Volume:  75     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-05-21     Completed Date:  2010-09-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1133-44     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences and the Alberta Ingenuity Centre for Carbohydrate Science, University of Alberta, Edmonton, Alberta T6G 2R3, Canada.
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MeSH Terms
Descriptor/Qualifier:
Acetylglucosamine / metabolism*
Anthraquinones / metabolism
Anti-Bacterial Agents / metabolism
Bacterial Proteins / genetics,  metabolism*
Gene Deletion
Gene Expression Regulation, Bacterial*
Membrane Transport Proteins / genetics,  metabolism*
Phosphates / metabolism
Phosphoenolpyruvate / metabolism
Signal Transduction
Streptomyces coelicolor / growth & development,  metabolism,  physiology*
Trans-Activators / metabolism
Transcription Factors / metabolism
Chemical
Reg. No./Substance:
0/Anthraquinones; 0/Anti-Bacterial Agents; 0/Bacterial Proteins; 0/Membrane Transport Proteins; 0/Phosphates; 0/Trans-Activators; 0/Transcription Factors; 1397-77-9/actinorhodin; 73-89-2/Phosphoenolpyruvate; 7512-17-6/Acetylglucosamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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