Document Detail

The permeability transition pore as a pathway for the release of mitochondrial DNA.
MedLine Citation:
PMID:  15808887     Owner:  NLM     Status:  MEDLINE    
This study shows that under oxidative stress DNA from liver mitochondria (mtDNA) can be released through the non-specific permeability transition pore. Pore opening was induced after the addition of Fe2+ and hydrogen peroxide, in the presence of calcium ions. Under these conditions mitochondria undergo large extent swelling, accompanied by the generation of thiobarbituric acid-reactive substances. It was observed that mtDNA was hydrolyzed after the oxidative stress, and it is proposed that some of the fragments were released from the matrix, in such a way that approximately 12% of the total mtDNA remained in the mitochondria. The remaining genetic material was analyzed, after its extraction in an agarose gel. The fragments released were smaller that 1000 bp, by analysis in a native 8% polyacrilamide gel. The presence of cyclosporin A, that inhibited permeability transition, also inhibited mtDNA release by roughly 52%.
Noemí García; José J García; Francisco Correa; Edmundo Chávez
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Life sciences     Volume:  76     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-05     Completed Date:  2005-05-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  2873-80     Citation Subset:  IM    
Departamento de Bioquímica, Instituto Nacional de Cardiología, Ignacio Chávez, Juan Badiano #1, México DF 014080, Mexico.
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MeSH Terms
Adenylate Kinase / metabolism
Biological Transport / drug effects,  physiology
Cyclosporine / pharmacology
DNA, Mitochondrial / metabolism*
Electrophoresis, Agar Gel
Hydrogen Peroxide / toxicity
Iron / toxicity
Lipid Peroxidation / drug effects
Mitochondria, Liver / metabolism*
Mitochondrial Swelling / drug effects*
Oxidative Stress / drug effects*,  physiology
Thiobarbituric Acid Reactive Substances / metabolism
Reg. No./Substance:
0/DNA, Mitochondrial; 0/Thiobarbituric Acid Reactive Substances; 59865-13-3/Cyclosporine; 7439-89-6/Iron; 7722-84-1/Hydrogen Peroxide; EC Kinase

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