Document Detail


The periosteum as a cellular source for functional tissue engineering.
MedLine Citation:
PMID:  19207046     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The periosteum, a specialized fibrous tissue composed of fibroblast, osteoblast, and progenitor cells, may be an optimal cell source for tissue engineering based on its accessibility, the ability of periosteal cells to proliferate rapidly both in vivo and in vitro, and the observed differentiation potential of these cells. However, the functional use of periosteum-derived cells as a source for tissue engineering requires an understanding of the ability of such cells to elaborate matrix of different tissues. In this study, we subjected a population of adherent primary periosteum-derived cells to both adipogenic and osteogenic culture conditions. The commitment propensity of periosteal cells was contrasted with that of well-characterized phenotypically pure populations of NIH3T3 fibroblast and MC3T3-E1 osteoblast cell lines. Our results demonstrate that the heterogeneous populations of periosteal cells and NIH3T3 fibroblasts have the ability to express both osteoblast-like and adipocyte-like markers with similar potential. This raises the question of whether fibroblasts within the periosteum may, in fact, have the potential to behave like progenitor cells and play a role in the tissue's multilineage potential or whether there are true stem cells within the periosteum. Further, this study suggests that expanded periosteal cultures may be a source for tissue engineering applications without extensive enrichment or sorting by molecular markers. Thus, this study lays the groundwork for future investigations that will more deeply enumerate the cellular sources and molecular events governing periosteal cell differentiation.
Authors:
Emily J Arnsdorf; Luis M Jones; Dennis R Carter; Christopher R Jacobs
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  15     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-28     Completed Date:  2009-10-27     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2637-42     Citation Subset:  IM    
Affiliation:
Bone and Joint R&D Center, VA Palo Alto Health Care System, Palo Alto, California, USA. emily.arnsdorf@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Adipogenesis
Alkaline Phosphatase / metabolism
Animals
Cell Lineage
Fibroblasts / cytology,  enzymology
Mice
NIH 3T3 Cells
Osteoblasts / cytology,  enzymology
Periosteum / cytology*
Tissue Engineering*
Grant Support
ID/Acronym/Agency:
AR45989/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
EC 3.1.3.1/Alkaline Phosphatase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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