Document Detail


The perinatal transition of the circulating metabolome in a nonhuman primate.
MedLine Citation:
PMID:  22391633     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: The fetal-to-neonatal transition is one of the most complex processes in biological existence; much is unknown about this transition on the molecular and biochemical level. Based on growing metabolomics literature, we hypothesize that metabolomic analysis will reveal the key biochemical intermediates that change during the birth transition.
RESULTS: Using two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GC × GC-TOFMS), we identified 100 metabolites that changed during this transition. Of these 100 metabolites, 23 demonstrated significant change during the first 72 h. Of note, four intermediates of the tricarboxylic acid (TCA) cycle were identified (α-ketoglutaric acid, fumaric acid, malic acid, and succinyl-CoA), demonstrating a consistent rate of rise during the study. This may signify the transition of the neonate from a hypoxic in utero environment to an oxygen-rich environment. Important signaling molecules were also identified, including myo-inositol and glutamic acid.
DISCUSSION: GC × GC-TOFMS was able to identify important metabolites associated with metabolism and signaling. These data can be used as a baseline for normal birth transition, which may aid in future perinatal research investigations.
METHODS: Late-preterm Macaca nemestrina were delivered by hysterotomy, with plasma drawn from the cord blood and after birth at eight additional time points to 72 h of age.
Authors:
Andrew C Beckstrom; Pattaraporn Tanya; Elizabeth M Humston; Laura R Snyder; Robert E Synovec; Sandra E Juul
Related Documents :
12620363 - [13c]-specific labeling of 8-2' linked (-)-cis-blechnic, (-)-trans-blechnic and (-)-bra...
22093753 - Neuroprotective activity of galloylated cyanogenic glucosides and hydrolysable tannins ...
1510793 - Synthesis and evaluation of antimicrobial activity of new 4-nitroso and 4-diazopyrazole...
Publication Detail:
Type:  Journal Article; Research Support, American Recovery and Reinvestment Act; Research Support, N.I.H., Extramural     Date:  2012-02-15
Journal Detail:
Title:  Pediatric research     Volume:  71     ISSN:  1530-0447     ISO Abbreviation:  Pediatr. Res.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-06     Completed Date:  2012-07-16     Revised Date:  2013-03-06    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  338-44     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, University of Washington, Seattle, Washington, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Carbon Dioxide / chemistry
Chromatography, Gas / methods
Citric Acid Cycle
Female
Genomics
Hydrogen-Ion Concentration
Macaca nemestrina
Mass Spectrometry / methods
Metabolome
Metabolomics / methods*
Oxidation-Reduction
Oxygen / metabolism
Parturition
Signal Transduction
Time Factors
Grant Support
ID/Acronym/Agency:
P30 HD02274/HD/NICHD NIH HHS; R01-HD-52820-01/HD/NICHD NIH HHS; UL1 TR000423/TR/NCATS NIH HHS
Chemical
Reg. No./Substance:
124-38-9/Carbon Dioxide; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Multidose optimization simulation of erythropoietin treatment in preterm infants.
Next Document:  Limited predictive value of early changes in EEG spectral power for neural injury after asphyxia in ...