Document Detail


The pericentromeric 21 DNA marker pGSM21 (D21S13) contains an expressed HTF island.
MedLine Citation:
PMID:  1692297     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The DNA marker locus D21S13, localized in the 21q11.1-q21 region, has been closely linked to familial Alzheimer's disease. We constructed a physical map of 1.7 Mb around D21S13 using probes pGSM21 and pGSE9. The results indicated that pGSM21 contains recognition sites for at least three rare-cutting restriction enzymes. The clustering of rare-cutting restriction sites is indicative of the presence of an HTF (HpaII tiny fragment) island. Restriction site mapping and methylation analysis proved that pGSM21 contains a methylation-free HTF island. Furthermore, a cDNA correlate has been isolated confirming that pGSM21 is part of an expressed sequence. Today, the gene associated with pGSM21 is the gene closest to the centromere on the 21q arm.
Authors:
P Stinissen; W Van Hul; G Van Camp; H Backhovens; A Wehnert; A Vandenberghe; C Van Broeckhoven
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genomics     Volume:  7     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  1990 May 
Date Detail:
Created Date:  1990-06-08     Completed Date:  1990-06-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  119-22     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Antwerp, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Chromosomes, Human, Pair 21*
DNA / genetics,  metabolism
Deoxyribonuclease HpaII
Deoxyribonucleases, Type II Site-Specific / metabolism*
Genes
Genetic Markers*
Humans
Male
Methylation
Restriction Mapping
Chemical
Reg. No./Substance:
0/Genetic Markers; 9007-49-2/DNA; EC 3.1.21.-/Deoxyribonuclease HpaII; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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