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The pericentriolar material in Chinese hamster ovary cells nucleates microtubule formation.
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MedLine Citation:
PMID:  559676     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The structure and function of the centrosomes from Chinese hamster ovary (CHO) cells were investigated by electron microscopy of negatively stained wholemount preparations of cell lysates. Cells were trypsinized from culture dishes, lysed with Triton X-100, sedimented onto ionized, carbon-coated grids, and negatively stained with phosphotungstate. The centrosomes from both interphase and dividing cells consisted of pairs of centrioles, a fibrous pericentriolar material, and a group of virus-like particles which were characteristic of the CHO cells and which served as markers for the pericentriolar material. Interphase centrosomes anchored up to two dozen microtubules when cells were lysed under conditions which preserved native microtubules. When Colcemid-blocked mitotic cells, initially devoid of microtubules, were allowed to recover for 10 min, microtubules formed at the pericentriolar material, but not at the centrioles. When lysates of Colcemid-blocked cells were incubated in vitro with micotubule protein purified from porcine brain tissue, up to 250 microtubules assembled at the centrosomes, similar to the number of microtubules that would normally form at the centrosome during cell division. A few microtubules could also be assembled in vitro onto the ends of isolated centrioles from which the pericentriolar material had been removed, forming characteristic axoneme- like bundles. In addition, microtubules; were assembled onto fragments of densely staining, fibrous material which was tentatively identified as periocentriolar material by its association of CHO can initiate and anchor microtubules both in vivo and in vitro.
Authors:
R R Gould; G G Borisy
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  73     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1977 Jun 
Date Detail:
Created Date:  1977-08-25     Completed Date:  1977-08-25     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  601-15     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division* / drug effects
Cells, Cultured
Colchicine / pharmacology
Cricetinae
Female
Microtubules* / drug effects,  ultrastructure
Organoids* / physiology,  ultrastructure
Ovary / cytology*
Tubulin / biosynthesis
Chemical
Reg. No./Substance:
0/Tubulin; 64-86-8/Colchicine
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 6 Year: 1977
Volume: 73 Issue: 3
First Page: 601 Last Page: 615
ID: 2111414
Publisher Id: 733601
PubMed Id: 559676

The pericentriolar material in Chinese hamster ovary cells nucleates microtubule formation
RR Gould
GG Borisy


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