Document Detail


A perforin-like protein mediates disruption of the erythrocyte membrane during egress of Plasmodium berghei male gametocytes.
MedLine Citation:
PMID:  23461714     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Successful gametogenesis of the malaria parasite depends on egress of the gametocytes from the erythrocytes within which they developed. Egress entails rupture of both the parasitophorous vacuole membrane and the erythrocyte plasma membrane, and precedes the formation of the motile flagellated male gametes in a process called exflagellation. We show here that egress of the male gametocyte depends on the function of a perforin-like protein, PPLP2. A mutant of Plasmodium berghei lacking PPLP2 displayed abnormal exflagellation; instead of each male gametocyte forming eight flagellated gametes, it produced gametocytes with only one, shared thicker flagellum. Using immunofluorescence and transmission electron microscopy analysis, and phenotype rescue with saponin or a pore-forming toxin, we conclude that rupture of the erythrocyte membrane is blocked in the mutant. The parasitophorous vacuole membrane, on the other hand, is ruptured normally. Some mutant parasites are still able to develop in the mosquito, possibly because the vigorous motility of the flagellated gametes eventually leads to escape from the persisting erythrocyte membrane. This is the first example of a perforin-like protein in Plasmodium parasites having a role in egress from the host cell and the first parasite protein shown to be specifically required for erythrocyte membrane disruption during egress.
Authors:
Elena Deligianni; Rhiannon N Morgan; Lucia Bertuccini; Christine C Wirth; Natalie C Silmon de Monerri; Lefteris Spanos; Michael J Blackman; Christos Louis; Gabriele Pradel; Inga Siden-Kiamos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-03-20
Journal Detail:
Title:  Cellular microbiology     Volume:  15     ISSN:  1462-5822     ISO Abbreviation:  Cell. Microbiol.     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-07-15     Completed Date:  2014-01-30     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  100883691     Medline TA:  Cell Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1438-55     Citation Subset:  IM    
Copyright Information:
© 2013 John Wiley & Sons Ltd.
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MeSH Terms
Descriptor/Qualifier:
Animals
Erythrocyte Membrane / parasitology*
Erythrocytes / parasitology
Germ Cells / metabolism*
Male
Mice
Mice, Inbred Strains
Models, Animal
Perforin / metabolism*
Phenotype
Plasmodium berghei / drug effects,  metabolism*
Protozoan Proteins / metabolism*
Saponins / pharmacology
Sperm Motility / physiology
Sperm Tail / drug effects,  physiology,  ultrastructure
Grant Support
ID/Acronym/Agency:
MC_U117532063//Medical Research Council; //Medical Research Council
Chemical
Reg. No./Substance:
0/Protozoan Proteins; 0/Saponins; 126465-35-8/Perforin

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