| The peptide nucleic acids (PNAs): introduction to a new class of probes for chromosomal investigation. | |
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MedLine Citation:
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PMID: 15156326 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Peptide nucleic acids (PNAs) are synthetic DNA mimics in which the sugar phosphate backbone is replaced by repeating N-(2-aminoethyl) glycine units linked by an amine bond and to which the nucleobases are fixed. Peptide nucleic acids hybridize with complementary nucleic acids with remarkably high affinity and specificity, essentially because of their uncharged and flexible polyamide backbone. The unique physicochemical properties of PNAs have led to the development of a large variety of biological research assays, and, over the last few years, PNAs have proved their powerful usefulness in genetic and cytogenetic diagnostic procedures. Several sensitive and robust PNA-dependent methods have been designed for modulating polymerase chain reactions, detecting genomic mutation or capturing nucleic acids. The more recent applications of PNA involve their use as molecular hybridization probes. Thus, the in situ detection of several human chromosomes has been reported in various types of tissues. |
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Authors:
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Franck Pellestor; Petra Paulasova |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review Date: 2004-05-19 |
Journal Detail:
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Title: Chromosoma Volume: 112 ISSN: 0009-5915 ISO Abbreviation: Chromosoma Publication Date: 2004 Jun |
Date Detail:
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Created Date: 2004-06-18 Completed Date: 2005-01-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985138R Medline TA: Chromosoma Country: Germany |
Other Details:
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Languages: eng Pagination: 375-80 Citation Subset: IM |
Affiliation:
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CNRS UPR 1142, Institute of Human Genetics, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France. franck.pellestor@igh.cnrs.fr |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromosomes, Human* Humans Peptide Nucleic Acids / chemistry*, genetics* |
| Chemical | |
Reg. No./Substance:
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0/Peptide Nucleic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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