Document Detail


The pattern of growth hormone delivery to peripheral tissues determines insulin-like growth factor-1 and lipolytic responses in obese subjects.
MedLine Citation:
PMID:  19470622     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CONTEXT: It is unclear whether the pattern of GH delivery to peripheral tissues has important effects. OBJECTIVE: The aim of the study was to compare the effects of pulsatile vs. continuous administration of GH upon metabolic and IGF-I parameters in obese subjects. SETTING: The study was conducted at the General Clinical Research Center at the University of Michigan Medical Center. PARTICIPANTS: Four men and five women with abdominal obesity (body mass index, 33 +/- 3 kg/m(2); body fat, 40 +/- 3%) participated in the study. INTERVENTION: GH (0.5 mg/m(2) . d) was given iv for 3 d as: 1) continuous infusion (C); and 2) pulsatile boluses (P) (15% of the dose at 0700, 1300, and 1800 h and 55% at 2400 h). These trials were preceded by a basal period (B) when subjects received normal saline. MAIN OUTCOME MEASURES: Rate of lipolysis and hepatic glucose production were evaluated using stable isotope tracer techniques. The composite index of insulin sensitivity (Matsuda index) was assessed using oral glucose tolerance test. RESULTS: The increase in plasma IGF-I concentrations was greater (P < 0.05) with continuous GH infusion (211 +/- 31, 423 +/- 38, and 309 +/- 34 microg/liter for B, C, and P, respectively). Muscle IGF-I mRNA was significantly increased (P < 0.05) only after the continuous GH infusion (1.2 +/- 0.4, 4.4 +/- 1.3, and 2.3 +/- 0.6 arbitrary units, for B, C, and P, respectively). Only pulsatile GH augmented the rate of lipolysis (4.1 +/- 0.3, 4.8 +/- 0.7, and 7.1 +/- 1.1 mumol/kg . min for B, C, and P, respectively). GH had no effect on hepatic glucose production, but both modes of GH administration were equally effective in impairing insulin sensitivity. CONCLUSION: These findings indicate that, in obese subjects, discrete components of GH secretory pattern may differentially affect IGF-I generation and lipolytic responses.
Authors:
Sowmya Surya; Jeffrey F Horowitz; Naila Goldenberg; Alla Sakharova; Matthew Harber; Andrea S Cornford; Kathy Symons; Ariel L Barkan
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-05-26
Journal Detail:
Title:  The Journal of clinical endocrinology and metabolism     Volume:  94     ISSN:  1945-7197     ISO Abbreviation:  J. Clin. Endocrinol. Metab.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-06     Completed Date:  2009-08-25     Revised Date:  2010-09-27    
Medline Journal Info:
Nlm Unique ID:  0375362     Medline TA:  J Clin Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2828-34     Citation Subset:  AIM; IM    
Affiliation:
Division of Metabolism, Endocrinology and Diabetes, University of Michigan, 3920 Taubman Center, Ann Arbor, MI 48109-5354, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Female
Glucose / metabolism
Human Growth Hormone / blood,  secretion*
Humans
Insulin-Like Growth Factor I / analysis*
Lipolysis*
Male
Muscle, Skeletal / chemistry
Obesity / metabolism*
Sex Characteristics
Grant Support
ID/Acronym/Agency:
R01 DK 071955/DK/NIDDK NIH HHS; UL1 RR 024986/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
12629-01-5/Human Growth Hormone; 50-99-7/Glucose; 67763-96-6/Insulin-Like Growth Factor I
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