Document Detail

The pattern of X-chromosome inactivation in the embryonic and extra-embryonic tissues of post-implantation digynic triploid LT/Sv strain mouse embryos.
MedLine Citation:
PMID:  2272499     Owner:  NLM     Status:  MEDLINE    
Spontaneously cycling LT/Sv strain female mice were mated to hemizygous Rb(X.2)2Ad males in order to facilitate the distinction of the paternal X chromosome, and the pregnant females were autopsied at about midday on the tenth day of gestation. Out of a total of 222 analysable embryos recovered, 165 (74.3%) were diploid and 57 (25.7%) were triploid. Of the triploids, 26 had an XXY and 31 an XXX sex chromosome constitution. Both embryonic and extra-embryonic tissue samples from the triploids were analysed cytogenetically by G-banding and by the Kanda technique to investigate their X-inactivation pattern. The yolk sac samples were separated enzymatically into their endodermally-derived and mesodermally-derived components, and these were similarly analysed, as were similar samples from a selection of control XmXp diploid embryos. In the case of the XmXmY digynic triploid embryos, a single darkly-staining Xm chromosome was observed in 485 (82.9%) out of 585, 304 (73.3%) out of 415, and 165 (44.7%) out of 369 metaphases from the embryonic, yolk sac mesodermally-derived and yolk sac endodermally-derived tissues, respectively. The absence of a darkly staining X-chromosome in the other metaphase spreads could either indicate that both X-chromosomes present were active, or that the Kanda technique had failed to differentially stain the inactive X-chromosome(s) present. In the case of the XmXmXp digynic triploid embryos, virtually all of the tissues analysed comprised two distinct cell lineages, namely those with two darkly-staining X-chromosomes, and those with a single darkly staining X-chromosome.(ABSTRACT TRUNCATED AT 250 WORDS)
S Speirs; J M Cross; M H Kaufman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genetical research     Volume:  56     ISSN:  0016-6723     ISO Abbreviation:  Genet. Res.     Publication Date:    1990 Oct-Dec
Date Detail:
Created Date:  1991-02-22     Completed Date:  1991-02-22     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  0370741     Medline TA:  Genet Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  107-14     Citation Subset:  IM    
Department of Anatomy, University Medical School, Edinburgh, UK.
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MeSH Terms
Chromosome Banding
Dosage Compensation, Genetic*
Embryo, Mammalian / physiology*
Embryonic Development
Embryonic and Fetal Development / genetics
X Chromosome*
Grant Support
//Wellcome Trust

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