Document Detail


The pattern of CagA and VacA proteins in Helicobacter pylori seropositive asymptomatic children in western Saudi Arabia.
MedLine Citation:
PMID:  16155650     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: This study aimed to determine antibody prevalence to Helicobacter pylori (H. pylori) virulence factors CagA and VacA in asymptomatic H. pylori seropositive children in Jeddah, Kingdom of Saudi Arabia (KSA). The possibility of differences in antibodies response patterns was also investigated in relation to gender, nationality and age. METHODS: Two hundred and twenty-four asymptomatic H. pylori seropositive children (mean age 9.3 +/- 3.9 years; range, 1-14 years) were enrolled in this study from King Abdul-Aziz University and Maternity and Children's Hospitals, Jeddah, KSA, during the periods 2002-2003. The 35 kDa, CagA or VacA H. pylori antibodies were measured in the serum by immunoblot (Helico Blot 2) method. RESULTS: Immunoblot assay yielded positive results in 215/224 seropositive asymptomatic children (96%). In those children, the prevalence of 35 kDa was 63.3%, VacA was 60%, CagA was 56.7% and for combined VacA and CagA antibodies was 45.6%. Prevalence of these bands did not show any difference between Saudi and non-Saudi children. Meanwhile, prevalence of 35 kDa, VacA, CagA, combined VacA and CagA antibodies were significantly elevated in males versus females (p<0.0001) and in children 10 years versus those in age groups 1-5 years and 6-9 years (p<0.0001). CONCLUSION: This study showed high prevalence of H. pylori antibodies among asymptomatic children in Jeddah, KSA. The prevalence of antibodies against 35 kDa, CagA and VacA H. pylori antigens is higher in males and older children. The ELISA and immunoblot are non-invasive methods that were found to have adequate performance in pediatric population.
Authors:
Soad M Jaber
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Saudi medical journal     Volume:  26     ISSN:  0379-5284     ISO Abbreviation:  Saudi Med J     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-09-12     Completed Date:  2007-09-19     Revised Date:  2008-06-23    
Medline Journal Info:
Nlm Unique ID:  7909441     Medline TA:  Saudi Med J     Country:  Saudi Arabia    
Other Details:
Languages:  eng     Pagination:  1372-7     Citation Subset:  IM    
Affiliation:
Pediatrics Consultant, Department of Pediatrics, King Abdul-Aziz University Hospital, PO Box 17818, Jeddah 21494, Kingdom of Saudi Arabia. soad_jaber@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Age Distribution
Antibodies, Bacterial / analysis,  immunology*
Antigens, Bacterial / analysis,  immunology*
Bacterial Proteins / analysis,  immunology*
Biological Markers / blood
Chi-Square Distribution
Child
Child, Preschool
Cohort Studies
Enzyme-Linked Immunosorbent Assay
Female
Helicobacter Infections / diagnosis,  epidemiology*,  immunology*
Helicobacter pylori / immunology,  pathogenicity*
Humans
Immunoblotting
Infant
Male
Probability
Saudi Arabia / epidemiology
Sensitivity and Specificity
Serologic Tests / methods
Severity of Illness Index
Sex Distribution
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/Bacterial Proteins; 0/Biological Markers; 0/VacA protein, Helicobacter pylori; 0/cagA protein, Helicobacter pylori

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